NKRF Polyclonal antibody

• Featured Product
• KD/KO Validated

NKRF Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14693-1-AP

Synonyms

ITBA4, NF kappa B repressing factor, NFKB repressing factor, NKRF, NRF, Protein ITBA4, Transcription factor NRF

---

Validation Data Gallery

• 

  WB analysis using 14693-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14693-1-AP (NKRF antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14693-1-AP

  WB result of NKRF antibody (14693-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKRF transfected HEK-293 cells.

• 

  IP experiment of HeLa using 14693-1-AP

  IP result of anti-NKRF (IP:14693-1-AP, 4ug; Detection:14693-1-AP 1:10000) with HeLa cells lysate 840 ug.

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

14693-1-AP targets NKRF in WB, IP, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NKRF fusion protein Ag6252
• Full Name: NFKB repressing factor
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC068514
• Gene Symbol: NKRF
• Gene ID (NCBI): 55922
• RRID: AB_2151628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NKRF, also named as Protein ITBA4 or Transcription factor NRF, is a 690 amino acid protein, which contains one G-patch domain and one R3H domain. NKRF is Widely and constitutively expressed, with lower level in colon, peripheral blood lymphocytes, lung and kidney. NKRF interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. NKRF is involved in the constitutive silencing of the IFN-β promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity.

Publications

Species	Application	Title
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin ; KD Validated
mouse	WB	Immunology; Down-regulation of miR-301a suppresses pro-inflammatory cytokines in TLR-triggered macrophages.; Authors - Huang Lisong L ; KD Validated
mouse	WB,IF,CoIP,ChIP	Adv Sci (Weinh); NKRF in Cardiac Fibroblasts Protects against Cardiac Remodeling Post-Myocardial Infarction via Human Antigen R; Authors - Chenghu Guo
human	WB	Int J Mol Sci; A1CF Binding to the p65 Interaction Site on NKRF Decreased IFN-β Expression and p65 Phosphorylation (Ser536) in Renal Carcinoma Cells; Authors - Yamin Liu