NKp46/NCR1 Recombinant monoclonal antibody

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  WB analysis of mouse splenocytes using 84416-5-RR

  mouse splenocytes were subjected to SDS PAGE followed by western blot with 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat spleen using 84416-5-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 84416-5-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat thymus using 84416-5-RR

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 84416-5-RR (NKp46/NCR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat spleen using 84416-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using NKp46/NCR1 antibody (84416-5-RR, Clone: 241811H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat spleen using 84416-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using NKp46/NCR1 antibody (84416-5-RR, Clone: 241811H7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 84416-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NKp46/NCR1 antibody (84416-5-RR, Clone: 241811H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84416-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84416-5-RR against Rat NKp46/NCR1 were performed. The affinity constant is 0.593 nM.

NKp46/NCR1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 84416-5-RR

Clone No.241811H7

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

241811H7, Activating receptor 1, Ar1, CD335, Kilr1

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Tested Applications

• Positive WB detected in: mouse splenocytes
• Positive IHC detected in: rat spleen tissue, rat thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat spleen tissue, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84416-5-RR targets NKp46/NCR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1469

  Product name: Recombinant Rat NKp46/NCR1 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 17-258 aa of NM_057199.1

  Sequence: QRINTEKQTLPKPIIWAKPSIMVTKGNSVNIWCQGAQSASEYQLYFEGSFFALERPKSSRSMNKVKFFISQMTSHTAGIYTCFYQSGELWSESSNPLKLVVTGLYDTPTLWVHPGPEVTLGENVTFSCHLKTATSKFFLLKERESNHIQHKYGNIQAEFPMGPVTRAHRGTYRCFGSYNDYAWSFPSEPVTLLITGEVENTSLAPTDPVSSLDYWEFDLSTKESGLQKDSAFWDHTAQNLIR

• Full Name: natural cytotoxicity triggering receptor 1
• Calculated Molecular Weight: 37kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: NM_057199.1
• Gene Symbol: NKp46/NCR1
• Gene ID (NCBI): 117547
• RRID: AB_3671945
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Z0H5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets, and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).