CoraLite® Plus 647 Anti-Rat NKp46/NCR1 Rabbit Recombinant Antibody

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• CoraLite® Plus 647 Anti-Rat NKp46/NCR1 Rabbit Recombinant Antibody (CL647-98250)

NKp46/NCR1 Uni-rAb^® Recombinant Antibody for FC

Cat No. CL647-98250 Copied!

Clone No. 241811E9

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Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC

Synonyms

241811E9, Activating receptor 1, Ar1, CD335, Kilr1

• 

  FC experiment of rat splenocytes using CL647-98250

  1x10^6 rat splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Rat NKp46/NCR1 Rabbit RecAb (CL647-98250, Clone: 241811E9) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: rat splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98250 targets NKp46/NCR1 in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1469

  Product name: Recombinant Rat NKp46/NCR1 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 17-258 aa of NM_057199.1

  Sequence: QRINTEKQTLPKPIIWAKPSIMVTKGNSVNIWCQGAQSASEYQLYFEGSFFALERPKSSRSMNKVKFFISQMTSHTAGIYTCFYQSGELWSESSNPLKLVVTGLYDTPTLWVHPGPEVTLGENVTFSCHLKTATSKFFLLKERESNHIQHKYGNIQAEFPMGPVTRAHRGTYRCFGSYNDYAWSFPSEPVTLLITGEVENTSLAPTDPVSSLDYWEFDLSTKESGLQKDSAFWDHTAQNLIR

• Full Name: natural cytotoxicity triggering receptor 1
• Calculated Molecular Weight: 37kDa
• GenBank Accession Number: NM_057199.1
• Gene Symbol: NKp46/NCR1
• Gene ID (NCBI): 117547
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Z0H5
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).