NLE1 Polyclonal antibody

NLE1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16419-1-AP

Synonyms

Nle, NLE1, Notchless protein homolog 1

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Validation Data Gallery

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  WB analysis of BxPC-3 using 16419-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16419-1-AP (NLE1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16419-1-AP targets NLE1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NLE1 fusion protein Ag9604
• Full Name: notchless homolog 1 (Drosophila)
• Calculated Molecular Weight: 485 aa, 53 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC012075
• Gene Symbol: NLE1
• Gene ID (NCBI): 54475
• RRID: AB_2878256
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.