NLRP10 Recombinant monoclonal antibody, PBS Only

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  WB analysis of mouse heart using 83131-8-RR (same clone as 83131-8-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 83131-8-RR (NLRP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83131-8-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83131-8-RR (same clone as 83131-8-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Nlrp10 Recombinant Antibody (83131-8-RR, Clone:230402C1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83131-8-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83131-8-RR

  Biolayer interferometry (BLl) kinetic assays of 83131-8-RR against Human NLRP10 were performed. The affinity constant is 0.899 nM.

NLRP10 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 83131-8-PBS

Clone No.230402C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

230402C1, CLR11.1, NACHT, LRR and PYD domains-containing protein 10, NALP10, NOD8

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Product Information

83131-8-PBS targets NLRP10 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag26402

  Product name: Recombinant human NLRP10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-180 aa of BC104957

  Sequence: MAMAKARKPREALLWALSDLEENDFKKLKFYLRDMTLSEGQPPLARGELEGLIPVDLAELLISKYGEKEAVKVVLKGLKVMNLLELVDQLSHICLHDYREVYREHVRCLEEWQEAGVNGRYNQVLLVAKPSSESPESLACPFPEQELESVTVEALFDSGEKPSLAPSLVVLQGSAGTGKT

• Full Name: NLR family, pyrin domain containing 10
• Calculated Molecular Weight: 655 aa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC104957
• Gene Symbol: NLRP10
• Gene ID (NCBI): 338322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q86W26
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NLRP10 is a member of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). NLRP10 likely plays a regulatory role in the innate immune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5.