Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, HepG2 cells, mouse skeletal muscle tissue, MCF-7 cells, HeLa cells, mouse colon tissue, mouse heart tissue, THP-1 cells, rat colon tissue, rat heart tissue
|Positive IP detected in
|mouse heart tissue
|Positive IHC detected in
|human heart tissue, human liver tissue, mouse heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:10-1:100
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
17215-1-AP targets NLRX1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|NLRX1 fusion protein Ag11000
|NLR family member X1
|Calculated molecular weight
|975 aa, 108 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
|Product Specific Protocols
|WB protocol for NLRX1 antibody 17215-1-AP
|IHC protocol for NLRX1 antibody 17215-1-AP
|IF protocol for NLRX1 antibody 17215-1-AP
|IP protocol for NLRX1 antibody 17215-1-AP
|Click here to view our Standard Protocols
Mitochondrial DNA stress primes the antiviral innate immune response.
Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1.
J Exp Med
NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity.
NLRX1 Facilitates Histoplasma capsulatum-Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages.