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  • KD/KO Validated

NLRX1 Polyclonal antibody

NLRX1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17215-1-AP

Synonyms

Caterpiller protein 11.3, CLR11.3, DLNB26, NLR family member X1, NLRX1, NOD26, NOD5, NOD9



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, mouse skeletal muscle tissue, MCF-7 cells, HeLa cells, mouse colon tissue, mouse heart tissue, THP-1 cells, rat colon tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human liver tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17215-1-AP targets NLRX1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NLRX1 fusion protein Ag11000
Full Name NLR family member X1
Calculated molecular weight 975 aa, 108 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC013199
Gene symbol NLRX1
Gene ID (NCBI) 79671
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NLRX1 antibody 17215-1-APDownload protocol
IHC protocol for NLRX1 antibody 17215-1-APDownload protocol
IF protocol for NLRX1 antibody 17215-1-APDownload protocol
IP protocol for NLRX1 antibody 17215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Innate Immun

NLRX1 does not inhibit MAVS-dependent antiviral signalling.

Authors - Fraser Soares
  • KO Validated
mouseWB,IF

Toxicol Appl Pharmacol

PMID: 29454061

Authors - Haiyan Yin
humanWB,IF,IP

In Vitro Cell Dev Biol Anim

The interaction of O-GlcNAc-modified NLRX1 and IKK-α modulates IL-1β expression in M1 macrophages.

Authors - Liqiong Chen
humanWB,CoIP

Sci Prog

NLRX1 can counteract innate immune response induced by an external stimulus favoring HBV infection by competitive inhibition of MAVS-RLRs signaling in HepG2-NTCP cells.

Authors - Qian Jiao
  • KD Validated
mouseWB

Mol Cell Biochem

The mitochondrial Nod-like receptor NLRX1 modifies apoptosis through SARM1.

Authors - Samuel A Killackey
humanWB,IF

Virus Res

Nucleotide-binding oligomerization domain-like receptor X1 restricts porcine reproductive and respiratory syndrome virus-2 replication by interacting with viral Nsp9.

Authors - Huiyuan Jing
  • KD Validated