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  • KD/KO Validated

NLRX1 Polyclonal antibody

NLRX1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17215-1-AP

Synonyms

Caterpiller protein 11.3, CLR11.3, DLNB26, NLR family member X1, NLRX1, NOD26, NOD5, NOD9



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, mouse skeletal muscle tissue, MCF-7 cells, HeLa cells, mouse colon tissue, mouse heart tissue, THP-1 cells, rat colon tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human liver tissue, mouse heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17215-1-AP targets NLRX1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NLRX1 fusion protein Ag11000
Full Name NLR family member X1
Calculated molecular weight 975 aa, 108 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC013199
Gene symbol NLRX1
Gene ID (NCBI) 79671
RRIDAB_2236031
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NLRX1 antibody 17215-1-APDownload protocol
IHC protocol for NLRX1 antibody 17215-1-APDownload protocol
IF protocol for NLRX1 antibody 17215-1-APDownload protocol
IP protocol for NLRX1 antibody 17215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Mitochondrial DNA stress primes the antiviral innate immune response.

Authors - A Phillip West
humanWB

Mol Cell

Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1.

Authors - Samuel A Killackey
  • KD Validated
humanWB,IHC

J Exp Med

NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity.

Authors - Geurt Stokman
  • KO Validated
human,mouseWB

PLoS Pathog

PMID: 28542569

Authors - Jae-Hoon Kim
humanWB

Front Immunol

PMID: 30344524

Authors - Tünde Fekete
mouseIF

Front Immunol

NLRX1 Facilitates Histoplasma capsulatum-Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages.

Authors - Juin-Hua Huang