Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HepG2 cells, mouse skeletal muscle tissue, MCF-7 cells, HeLa cells, mouse colon tissue, mouse heart tissue, THP-1 cells, rat colon tissue, rat heart tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human heart tissue, human liver tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17215-1-AP targets NLRX1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NLRX1 fusion protein Ag11000|
|Full Name||NLR family member X1|
|Calculated molecular weight||975 aa, 108 kDa|
|Observed molecular weight||100-110 kDa|
|GenBank accession number||BC013199|
|Gene ID (NCBI)||79671|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
|Product Specific Protocols|
|WB protocol for NLRX1 antibody 17215-1-AP||Download protocol|
|IHC protocol for NLRX1 antibody 17215-1-AP||Download protocol|
|IF protocol for NLRX1 antibody 17215-1-AP||Download protocol|
|IP protocol for NLRX1 antibody 17215-1-AP||Download protocol|
|Click here to view our Standard Protocols|
NLRX1 does not inhibit MAVS-dependent antiviral signalling.
Toxicol Appl Pharmacol
In Vitro Cell Dev Biol Anim
The interaction of O-GlcNAc-modified NLRX1 and IKK-α modulates IL-1β expression in M1 macrophages.
NLRX1 can counteract innate immune response induced by an external stimulus favoring HBV infection by competitive inhibition of MAVS-RLRs signaling in HepG2-NTCP cells.
Mol Cell Biochem
The mitochondrial Nod-like receptor NLRX1 modifies apoptosis through SARM1.
Nucleotide-binding oligomerization domain-like receptor X1 restricts porcine reproductive and respiratory syndrome virus-2 replication by interacting with viral Nsp9.