NMDAR1/GRIN1 Recombinant antibody

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  WB analysis using 85973-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85973-3-RR (NMDAR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 85973-3-RR

  IP result of anti-NMDAR1/GRIN1 (IP:85973-3-RR, 4ug; Detection:85973-3-RR 1:1000) with mouse brain tissue lysate 1720 ug.

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  Affinity and Kinetic Characterization of 85973-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85973-3-RR against Human NMDAR1/GRIN1 were performed. The affinity constant is below 1 pM.

NMDAR1/GRIN1 Uni-rAb^TM Recombinant Antibody for WB, IP, ELISA

Cat No. 85973-3-RR

Clone No.250535B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

GRIN1, NMDAR1, GluN1, Glutamate receptor ionotropic, NMDA 1, NMDAR 1

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85973-3-RR targets NMDAR1/GRIN1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NMDAR1/GRIN1 fusion protein Ag26093
• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 116-120 kDa
• GenBank Accession Number: NM_000832
• Gene Symbol: NMDAR1
• Gene ID (NCBI): 2902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q05586
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRIN1 encodes subunit 1 of the N-methyl-D-aspartate (NMDA) receptor, which is a heteromeric glutamate-gated calcium ion channel essential for synaptic function in the brain (PMID: 25864721, PMID: 25864721). NMDARs play important roles in normal brain development and function, such as synaptic plasticity, neural development, learning and memory (PMID: 20716669). NMDAR dysfunction has been associated with several neurological disorders including Parkinson, Alzheimer and Huntington diseases. Disrupted motor learning and long-term synaptic plasticity in mice lacking NMDAR1 in the striatum (PMID: 17015831).