Tested Applications
| Positive WB detected in | human adrenal gland tissue, human heart tissue, human liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13442-2-AP targets NNT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4259 Product name: Recombinant human NNT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-236 aa of BC032370 Sequence: MNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK Predict reactive species |
| Full Name | nicotinamide nucleotide transhydrogenase |
| Calculated Molecular Weight | 1085 aa, 114 kDa |
| Observed Molecular Weight | 114 kDa |
| GenBank Accession Number | BC032370 |
| Gene Symbol | NNT |
| Gene ID (NCBI) | 23530 |
| RRID | AB_2153596 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13423 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and βsubunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 110 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NNT antibody 13442-2-AP | Download protocol |
| IHC protocol for NNT antibody 13442-2-AP | Download protocol |
| IP protocol for NNT antibody 13442-2-AP | Download protocol |
| WB protocol for NNT antibody 13442-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NNT Polyclonal antibody (catalog number 13442-2-AP) has 25 citations. These appear in journals including Nature Metabolism, Nature Aging, Nature Cell Biology, Redox Biology, Science Advances, Cell Death & Disease, Phytomedicine, Archives of Toxicology, Free Radical Biology and Medicine, Cell Reports, Biochemical Pharmacology, Frontiers in Cell and Developmental Biology, Scientific Reports, FASEB Journal, Molecular Cell, and others. Research fields span mitochondrial metabolism, redox homeostasis, cancer, neurodegeneration, aging, diabetes, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Nat Aging Inhibiting bridge integrator 2 phosphorylation leads to improved oocyte quality, ovarian health and fertility in aging and after chemotherapy in mice | ||
Nat Cell Biol Mitochondrial NADPH fuels mitochondrial fatty acid synthesis and lipoylation to power oxidative metabolism | ||
Redox Biol Nicotinamide nucleotide transhydrogenase-mediated redox homeostasis promotes tumor growth and metastasis in gastric cancer.
| ||
Sci Adv Chronic stress hinders sensory axon regeneration via impairing mitochondrial cristae and OXPHOS | ||
Cell Death Dis ApoM maintains cellular homeostasis between mitophagy and apoptosis by affecting the stability of Nnt mRNA through the Zic3-ApoM-Elavl2-Nnt axis during neural tube closure |
