NNT Polyclonal antibody

• Featured Product
• KD/KO Validated

NNT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 13442-2-AP

Synonyms

EC:7.1.1.1

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Validation Data Gallery

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  WB analysis of human adrenal gland using 13442-2-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13442-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13442-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 13442-2-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13442-2-AP

  IP result of anti-NNT (IP:13442-2-AP, 4ug; Detection:13442-2-AP 1:300) with HepG2 cells lysate 2800ug.

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  IHC staining of human liver cancer using 13442-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13442-2-AP (NNT antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 13442-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13442-2-AP (NNT antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13442-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NNT antibody (13442-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13442-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13442-2-AP (NNT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human adrenal gland tissue, human heart tissue, human liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

13442-2-AP targets NNT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NNT fusion protein Ag4259
• Full Name: nicotinamide nucleotide transhydrogenase
• Calculated Molecular Weight: 1085 aa, 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: BC032370
• Gene Symbol: NNT
• Gene ID (NCBI): 23530
• RRID: AB_2153596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and βsubunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 110 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Redox Biol; Nicotinamide nucleotide transhydrogenase-mediated redox homeostasis promotes tumor growth and metastasis in gastric cancer.; Authors - Shuai Li ; KD Validated
rat	WB	Front Cell Dev Biol; Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.; Authors - Hao Zhang
mouse	WB	Sci Rep; Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.; Authors - Lei Wu
mouse	WB	Cell Physiol Biochem; Effect of Type I Diabetes on the Proteome of Mouse Oocytes.; Authors - Guangjian Jiang
human	WB	Mol Nutr Food Res; Lack of β, β-carotene -9', 10'-oxygenase 2 leads to hepatic mitochondrial dysfunction and cellular oxidative stress in mice.; Authors - Lei Wu
human	WB	Am J Med Genet A; Nicotinamide nucleotide transhydrogenase mutation analysis in Chinese patients with thyroid dysgenesis.; Authors - Miaomiao Li