NNT Polyclonal antibody, PBS Only

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  WB analysis of human adrenal gland using 13442-2-AP (same clone as 13442-2-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 13442-2-AP (same clone as 13442-2-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 13442-2-AP (same clone as 13442-2-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human adrenal gland using 13442-2-AP (same clone as 13442-2-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13442-2-AP (NNT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 13442-2-AP (same clone as 13442-2-PBS)

  IP result of anti-NNT (IP:13442-2-AP, 4ug; Detection:13442-2-AP 1:300) with HepG2 cells lysate 2800ug. This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 13442-2-AP (same clone as 13442-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13442-2-AP (NNT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 13442-2-AP (same clone as 13442-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13442-2-AP (NNT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13442-2-AP (same clone as 13442-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NNT antibody (13442-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13442-2-AP (same clone as 13442-2-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13442-2-AP (NNT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13442-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NNT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13442-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:7.1.1.1

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Product Information

13442-2-PBS targets NNT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4259

  Product name: Recombinant human NNT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 30-236 aa of BC032370

  Sequence: MNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

• Full Name: nicotinamide nucleotide transhydrogenase
• Calculated Molecular Weight: 1085 aa, 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: BC032370
• Gene Symbol: NNT
• Gene ID (NCBI): 23530
• RRID: AB_2153596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13423
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NNT(nicotinamide nucleotide transhydrogenase) is a transmembrane protein and functions as a proton pumping transhydrogenase. The protein is present in both prokaryotes and eukaryotes and is located in the inner membrane of mitochondria. In prokaryotic cells, the enzyme is composed of α and βsubunits of 54 and 48 kDa, respectively. In eukaryotic cells, the enzyme is usually composed of a single peptide of 110 kDa. Although NNT catalyzes the interconversion of NADH and NADPH, the forward reaction using the reducing power of NADH to regenerate NADPH would be favored under conditions of oxidative stress(PMID:16497723). It can exsit as a homodimer(PMID:21882037).