NOB1 Polyclonal antibody

NOB1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 14456-1-AP

Synonyms

ART 4, ART4, MST158, MSTP158, NOB1, NOB1P, Protein ART 4, PSMD8BP1, RNA binding protein NOB1



Tested Applications

Positive WB detected inJurkat cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

14456-1-AP targets NOB1 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOB1 fusion protein Ag6051
Full Name NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
Calculated molecular weight 46 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC064630
Gene symbol NOB1
Gene ID (NCBI) 28987
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for NOB1 antibody 14456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols