RPH3AL Polyclonal antibody

RPH3AL Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15297-1-AP

Synonyms

No C2 domains protein, NOC2, Rab effector Noc2, Rabphilin 3A like protein, RPH3AL

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Validation Data Gallery

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  WB analysis of human liver using 15297-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15297-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15297-1-AP

  IP result of anti-RPH3AL (IP:15297-1-AP, 4ug; Detection:15297-1-AP 1:500) with HepG2 cells lysate 2400ug.

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Tested Applications

• Positive WB detected in: human liver tissue, L02 cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15297-1-AP targets RPH3AL in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPH3AL fusion protein Ag7552
• Full Name: rabphilin 3A-like (without C2 domains)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC005153
• Gene Symbol: RPH3AL
• Gene ID (NCBI): 9501
• RRID: AB_2181270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rabphilin-3A-like (RPH3AL), also known as Noc2, is a Rab effector protein with a molecular weight of 38 kDa. It is expressed abundantly in endocrine cells but at low levels in exocrine tissues, playing a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells, and also having a key role in INS secretion by pancreatic cells. RPH3AL functions as a potential RAB3B effector protein in epithelial cells, and RPH3AL gene is likely a tumor suppressor.

Publications

Species	Application	Title
human	WB	J Cell Sci; Rab2a and Rab27a cooperatively regulate transition from granule maturation to exocytosis through the dual effector Noc2.; Authors - Kohichi Matsunaga
human	WB	Clin Chim Acta; Detection of autoantibodies against Rabphilin-3A-like protein as a potential biomarker in patient's sera of colorectal cancer.; Authors - Jinn-Shiun Chen