NOL9 Polyclonal antibody

• 

  WB analysis of HEK-293 using 16083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16083-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16083-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16083-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16083-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16083-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16083-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

NOL9 Polyclonal Antibody for WB, ELISA

Cat No. 16083-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA, FRAP

Synonyms

NOL9, nucleolar protein 9

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

16083-1-AP targets NOL9 in WB, IF, ELISA, FRAP applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9082

  Product name: Recombinant human NOL9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 82-430 aa of BC009257

  Sequence: TEPVLGPPFTHLRTPQKMVYYGKPSCKNNYENYIDIVKYVFSAYKRESPLIVNTMGWVSDQGLLLLIDLIRLLSPSHVVQFRSDHSKYMPDLTPQYVDDMDGLYTKSKTKMRNRRFRLAAFADALEFADEEKESPVEFTGHKLIGVYTDFAFRITPRNRESHNKILRDLSILSYLSQLQPPMPKPLSPLHSLTPYQVPFNAVALRITHSDVAPTHILYAVNASWVGLCKIQDDVRGYTNGPILLAQTPICDCLGFGICRGIDMEKRLYHILTPVPPEELRTVNCLLVGAIAIPHCVLKCQRGIEGTVPYVTTDYNFKLPGASEKIGAREPEEAHKEKPYRRPKFCRKMK

• Full Name: nucleolar protein 9
• Calculated Molecular Weight: 702 aa, 79 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC009257
• Gene Symbol: NOL9
• Gene ID (NCBI): 79707
• RRID: AB_11124314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5SY16
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOL9, also named as Polynucleotide 5'-hydroxyl-kinase NOL9, is a 702 amino acid protein, which belongs to the Clp1 family. NOL9 localizes in the nucleus and as a polynucleotide 5'-kinase is involved in rRNA processing. its polynucleotide kinase activity is required for efficient generation of the 5.8S and 28S rRNAs from the 32S precursor.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	FRAP	Nucleic Acids Res; Early rRNA processing is a stress-dependent regulatory event whose inhibition maintains nucleolar integrity.; Authors - Witold Szaflarski
human	WB,IF	Mol Biol Cell; LAS1L interacts with the mammalian Rix1 complex to regulate ribosome biogenesis.; Authors - Castle Christopher D CD
human	WB	Cancer Res Commun; USP36 SUMOylates Las1L and promotes its function in pre-rRNA ITS2 processing; Authors - Yanping Li