NOP58 Recombinant antibody

NOP58 Recombinant Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

230058C7

Cat no : 82879-1-RR

Synonyms

HSPC120, NOL5, NOP5, NOP5/NOP58, NOP58, Nucleolar protein 5, Nucleolar protein 58

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Validation Data Gallery

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  WB analysis using 82879-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82879-1-RR (NOP58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 82879-1-RR

  IP result of anti-NOP58 (IP:82879-1-RR, 4ug; Detection:82879-1-RR 1:2000) with HeLa cells lysate 1600 ug.

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  IF Staining of U2OS using 82879-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOP58 antibody (82879-1-RR) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82879-1-RR targets NOP58 in WB, IP, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NOP58 fusion protein Ag5740
• Full Name: NOP58 ribonucleoprotein homolog (yeast)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC032592
• Gene Symbol: NOP58
• Gene ID (NCBI): 51602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The box C/D snoRNPs are named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation (PMID:9649444,8878486). Nop58 is a component common to the box C/D small nucleolar ribonucleoprotein (PMID:10606270).