NOTCH2 Polyclonal antibody

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• KD/KO Validated

NOTCH2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 28580-1-AP

Synonyms

Notch homolog 2 (Drosophila), Notch 2, N2ICD, N2ECD, hN2

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Validation Data Gallery

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  WB analysis using 28580-1-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 28580-1-AP (NOTCH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 28580-1-AP

  WB result of NOTCH2 antibody (28580-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and NOTCH2 knockout HEK-293T cells.

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  IP experiment of U-87 MG using 28580-1-AP

  IP result of anti-NOTCH2 (IP:28580-1-AP, 4ug; Detection:28580-1-AP 1:5000) with U-87 MG cells lysate 1960 ug.

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  IP experiment of HEK-293 using 28580-1-AP

  IP result of anti-NOTCH2 (IP:28580-1-AP, 4ug; Detection:28580-1-AP 1:5000) with HEK-293 cells lysate 1575 ug.

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  IHC staining of human stomach using 28580-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28580-1-AP (NOTCH2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U-87 MG cells, HEK-293T cells
• Positive IP detected in: HEK-293 cells, U-87 MG cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Product Information

28580-1-AP targets NOTCH2 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOTCH2 fusion protein Ag29413
• Full Name: Notch homolog 2 (Drosophila)
• Calculated Molecular Weight: 265 kDa
• Observed Molecular Weight: 110 kDa, 265 kDa
• GenBank Accession Number: NM_024408
• Gene Symbol: NOTCH2
• Gene ID (NCBI): 4853
• RRID: AB_2881175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).

Publications

Species	Application	Title
human	WB	Cancer Lett; Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer.; Authors - Rucheng Yan
human	CoIP	Int J Mol Sci; APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.; Authors - Marianna Naki
human	WB	Life Sci; GALNT2 promotes cell proliferation, migration, and invasion by activating the Notch/Hes1-PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma.; Authors - Wei Wang
human	WB	Ann Transl Med; SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway.; Authors - Yan Feng
human	IHC	Transl Androl Urol; Evaluation of NOTCH family genes' expression and prognostic value in prostate cancer.; Authors - Yunhao Qing
human	WB	Front Oncol; A comprehensive role evaluation and mechanism exploration of POGLUT2 in pan-cancer; Authors - Xianyun Xu