NOTCH2 Polyclonal antibody

NOTCH2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 28580-1-AP

Synonyms

AGS2, hN2, Notch 2, Notch homolog 2 (Drosophila), NOTCH2

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Validation Data Gallery

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  WB analysis using 28580-1-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 28580-1-AP (NOTCH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

28580-1-AP targets NOTCH2 in WB, IHC, CoIP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOTCH2 fusion protein Ag29413
• Full Name: Notch homolog 2 (Drosophila)
• Calculated Molecular Weight: 265 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_024408
• Gene Symbol: NOTCH2
• Gene ID (NCBI): 4853
• RRID: AB_2881175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Lett; Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer.; Authors - Rucheng Yan
human	CoIP	Int J Mol Sci; APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.; Authors - Marianna Naki
human	WB	Life Sci; GALNT2 promotes cell proliferation, migration, and invasion by activating the Notch/Hes1-PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma.; Authors - Wei Wang
human	WB	Ann Transl Med; SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway.; Authors - Yan Feng
human	IHC	Transl Androl Urol; Evaluation of NOTCH family genes' expression and prognostic value in prostate cancer.; Authors - Yunhao Qing
human	WB	Front Oncol; A comprehensive role evaluation and mechanism exploration of POGLUT2 in pan-cancer; Authors - Xianyun Xu