NPC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NPC1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

22

Reviews (3)

Cat no : 13926-1-AP

Synonyms

NPC intracellular cholesterol transporter 1, Niemann-Pick C1 protein, Niemann Pick disease, type C1, Niemann Pick C1 protein

---

Validation Data Gallery

• 

  WB analysis using 13926-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13926-1-AP (NPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13926-1-AP

  WB result of NPC1 antibody (13926-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NPC1 transfected HEK-293 cells.

• 

  WB analysis of HepG2 using 13926-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13926-1-AP (NPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13926-1-AP

  unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 13926-1-AP (NPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13926-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13926-1-AP (NPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13926-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13926-1-AP (NPC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13926-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13926-1-AP (NPC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 13926-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13926-1-AP (NPC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 13926-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13926-1-AP (NPC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 13926-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13926-1-AP (NPC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of Neuro-2a using 13926-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using NPC1 antibody (13926-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 13926-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13926-1-AP (NPC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 13926-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NPC1 (13926-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: unboiled HEK-293 cells, HEK-293 cells, HepG2 cells, unboiled mouse brain tissue, HeLa cells
• Positive IHC detected in: human liver cancer tissue, human placenta tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Neuro-2a cells, HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 18 publications below
• IHC: See 4 publications below

Product Information

13926-1-AP targets NPC1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPC1 fusion protein Ag4946
• Full Name: Niemann-Pick disease, type C1
• Calculated Molecular Weight: 142 kDa
• Observed Molecular Weight: 160-200 kDa
• GenBank Accession Number: BC063302
• Gene Symbol: NPC1
• Gene ID (NCBI): 4864
• RRID: AB_2152050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Niemann-Pick Type C (NPC) disease is a lysosomal storage disorder characterized by the accumulation of unesterified cholesterol and other lipids in the endolysosomal system. NPC disease results from a defect in either of two distinct cholesterol-binding proteins: a transmembrane protein, NPC1, and a small soluble protein, NPC2. NPC1 or NPC2 deficiency models showed that the functions of these two proteins within lysosomes are linked closely. NPC1 is a typical transmembrane protein and contains a number of modification sites for glycosylation. Defects in NPC1 are the cause of Niemann-Pick disease type C1 which exhibits highly variable clinical phenotype. Moreover, NPC1 may play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals.

Publications

Species	Application	Title
human	WB	Cell; Cholesterol Transport through Lysosome-Peroxisome Membrane Contacts.; Authors - Bei-Bei Chu ; KD Validated
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
human	WB	Proc Natl Acad Sci U S A; Cholesterol trafficking is required for mTOR activation in endothelial cells.; Authors - Xu Jing J ; KD Validated
mouse	WB	Proc Natl Acad Sci U S A; Inhibition of sphingolipid synthesis improves outcomes and survival in GARP mutant wobbler mice, a model of motor neuron degeneration.; Authors - Constance S Petit
mouse	WB	Cell Rep; The GARP Complex Is Involved in Intracellular Cholesterol Transport via Targeting NPC2 to Lysosomes.; Authors - Jian Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Parijat (Verified Customer) (04-08-2024)

Good antibody for specific staining of NPC1 in IF.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: MEF

FH

Ganesh (Verified Customer) (02-07-2024)

25 µg of HAP1 (human) whole cell lysate was resolved on a 4-12% Bis-Tris Gel and proteins were transferred onto a nitrocellulose membrane. NPC1 antibody was diluted at 1:1000 in Blocking Buffer (5% Milk in 1x TBS-T (0.1% Tween-20)) and the membrane was incubated overnight at 4 deg C on a rotator.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HAP1

NPC1 Antibody Western Blot validation (1:1000 dilution) in HAP1 (Cat no:13926-1-AP)

FH

Benjamin (Verified Customer) (12-09-2021)

Nice antibody that detects Npc1 at the correct molecular weight in primary mouse neurons.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary Mouse Neurons

NPC1 Antibody Western Blot validation (1:1000 dilution) in Primary Mouse Neurons (Cat no:13926-1-AP)