Tested Applications
| Positive WB detected in | unboiled HEK-293 cells, HepG2 cells, unboiled mouse brain tissue, HEK-293 cells, 37℃ incubated HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human placenta tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13926-1-AP targets NPC1 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4946 Product name: Recombinant human NPC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-266 aa of BC063302 Sequence: QSCVWYGECGIAYGDKRYNCEYSGPPKPLPKDGYDLVQELCPGFFFGNVSLCCDVRQLQTLKDNLQLPLQFLSRCPSCFYNLLNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNVKELQYYVGQGFANAMYNACRDVEAPSSNDKALGLLCGKDADACNATNWIEYMFNKDNGQAPFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDCSIVCGPKPQPPPPPAPWTILGLD Predict reactive species |
| Full Name | Niemann-Pick disease, type C1 |
| Calculated Molecular Weight | 142 kDa |
| Observed Molecular Weight | 160-200 kDa |
| GenBank Accession Number | BC063302 |
| Gene Symbol | NPC1 |
| Gene ID (NCBI) | 4864 |
| RRID | AB_2152050 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15118 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Niemann-Pick Type C (NPC) disease is a lysosomal storage disorder characterized by the accumulation of unesterified cholesterol and other lipids in the endolysosomal system. NPC disease results from a defect in either of two distinct cholesterol-binding proteins: a transmembrane protein, NPC1, and a small soluble protein, NPC2. NPC1 or NPC2 deficiency models showed that the functions of these two proteins within lysosomes are closely linked. NPC1 is a typical transmembrane protein and contains a number of modification sites for glycosylation. Defects in NPC1 are the cause of Niemann-Pick disease type C1, which exhibits a highly variable clinical phenotype. Moreover, NPC1 may play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals.(PMID: 20525256; PMID: 31608980; PMID: 15465421)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NPC1 antibody 13926-1-AP | Download protocol |
| IHC protocol for NPC1 antibody 13926-1-AP | Download protocol |
| WB protocol for NPC1 antibody 13926-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NPC1 antibody (Cat# 13926-1-AP) has accumulated 33 citations in high-impact journals including Cell, Nature Communications, Molecular Cell, PNAS, EMBO Journal, Cancer Research, and Journal of Lipid Research. Associated research fields encompass cholesterol trafficking, lipid metabolism, mTOR signaling, viral entry mechanisms, Niemann-Pick disease, atherosclerosis, and oncology (hepatocellular, gastric, and ovarian cancers). Applications are predominantly Western blot and immunohistochemistry.
| Species | Application | Title |
|---|---|---|
Nat Commun A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry. | ||
Mol Cell mTOR inhibition reprograms cellular lipid homeostasis by inducing alternative lipid uptake and promoting cholesterol transport.
| ||
Cancer Lett Pharmacological blockade of cholesterol trafficking by cepharanthine in endothelial cells suppresses angiogenesis and tumor growth. | ||
Int J Biol Sci Astemizole Inhibits mTOR Signaling and Angiogenesis by Blocking Cholesterol Trafficking. | ||
Sci China Life Sci Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P2 and extended synaptotagmins. |
Reviews
What customers say
All three reviews are positive, with ratings of 5, 5, and 4 out of 5. Users report reliable and specific detection of NPC1 across multiple applications. One user found excellent specific staining in immunofluorescence on MEF cells at 1:200 dilution. Two users confirmed clear Western Blot results at 1:1000 dilution—one in HAP1 whole cell lysate and another in primary mouse neurons—both showing the band at the correct molecular weight. Overall, reviewers highlight the antibody's specificity, consistent performance, and suitability for both IF and WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Parijat (Verified Customer) (04-08-2024) | Good antibody for specific staining of NPC1 in IF.
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Ganesh (Verified Customer) (02-07-2024) | 25 µg of HAP1 (human) whole cell lysate was resolved on a 4-12% Bis-Tris Gel and proteins were transferred onto a nitrocellulose membrane. NPC1 antibody was diluted at 1:1000 in Blocking Buffer (5% Milk in 1x TBS-T (0.1% Tween-20)) and the membrane was incubated overnight at 4 deg C on a rotator.
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Benjamin (Verified Customer) (12-09-2021) | Nice antibody that detects Npc1 at the correct molecular weight in primary mouse neurons.
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