Nephrin Monoclonal antibody

Nephrin Monoclonal Antibody for IHC, ELISA

Cat No. 66970-1-Ig
Clone No.1D11H8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

NPHS1, 1D11H8, CNF, NPHN, NPHS 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66970-1-Ig targets Nephrin in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19273

Product name: Recombinant human NPHS1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 89-374 aa of BC156935

Sequence: PARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQTENKNVTLSCVSKSSRPRVLLRW

Predict reactive species
Full Name nephrosis 1, congenital, Finnish type (nephrin)
Calculated Molecular Weight 1241 aa, 135 kDa
GenBank Accession NumberBC156935
Gene Symbol Nephrin
Gene ID (NCBI) 4868
RRIDAB_2882292
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO60500
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).

Protocols

Product Specific Protocols
IHC protocol for Nephrin antibody 66970-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Phytomedicine

Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro

Authors - Zhongzhu Ai
WB,IF

Phytomedicine

Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis

Authors - Daofei Song
IF

Genomics

Effectiveness of a novel rat model of off-target PLA2R1 knockout to renal impairment

Authors - Bo Huang
humanWB

Mol Cell Endocrinol

Experimental study on renoprotective effect of intermedin on diabetic nephropathy.

Authors - Yanhong Wang
WB

Exp Mol Pathol

Urinary exosomes from patients with diabetic kidney disease induced podocyte apoptosis via microRNA-145-5p/Srgap2 and the RhoA/ROCK pathway

Authors - Lulu Han
WB,IHC,IF

Heliyon

QiDiTangShen granules alleviates diabetic nephropathy podocyte injury: A network pharmacology study and experimental validation in vivo and vitro

Authors - Fei Gao
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