Nephrin Monoclonal antibody

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  IHC staining of human kidney using 66970-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66970-1-Ig (Nephrin antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66970-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66970-1-Ig (Nephrin antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Nephrin Monoclonal Antibody for IHC, ELISA

Cat No. 66970-1-Ig

Clone No.1D11H8

Publications(16)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

NPHS1, 1D11H8, CNF, NPHN, NPHS 1

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Tested Applications

• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 10 publications below

Product Information

66970-1-Ig targets Nephrin in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Nephrin fusion protein Ag19273
• Full Name: nephrosis 1, congenital, Finnish type (nephrin)
• Calculated Molecular Weight: 1241 aa, 135 kDa
• GenBank Accession Number: BC156935
• Gene Symbol: Nephrin
• Gene ID (NCBI): 4868
• RRID: AB_2882292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O60500
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Phytomedicine; Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro; Authors - Zhongzhu Ai
	WB,IF	Phytomedicine; Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis; Authors - Daofei Song
	IF	Genomics; Effectiveness of a novel rat model of off-target PLA2R1 knockout to renal impairment; Authors - Bo Huang
human	WB	Mol Cell Endocrinol; Experimental study on renoprotective effect of intermedin on diabetic nephropathy.; Authors - Yanhong Wang
	WB	Exp Mol Pathol; Urinary exosomes from patients with diabetic kidney disease induced podocyte apoptosis via microRNA-145-5p/Srgap2 and the RhoA/ROCK pathway; Authors - Lulu Han
	WB,IHC,IF	Heliyon; QiDiTangShen granules alleviates diabetic nephropathy podocyte injury: A network pharmacology study and experimental validation in vivo and vitro; Authors - Fei Gao