Nephrin Polyclonal antibody

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• Nephrin Polyclonal antibody (22912-1-AP)

Nephrin Polyclonal Antibody for WB, ELISA

Cat No. 22912-1-AP Copied!

Publications (26)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Synonyms

NPHS1, CNF, NPHN, NPHS 1, Renal glomerulus-specific cell adhesion receptor

• 

  WB analysis using 22912-1-AP

  Mouse kidney tissues were subjected to SDS PAGE followed by western blot with 22912-1-AP (Nephrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22912-1-AP targets Nephrin in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18994

  Product name: Recombinant human NPHS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 89-374 aa of BC156935

  Sequence: PARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQTENKNVTLSCVSKSSRPRVLLRW

• Full Name: nephrosis 1, congenital, Finnish type (nephrin)
• Calculated Molecular Weight: 1241 aa, 135 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC156935
• Gene Symbol: Nephrin
• Gene ID (NCBI): 4868
• RRID: AB_3669406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: O60500
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is the podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).

Publications

What published studies show

The Nephrin Polyclonal antibody (Cat# 22912-1-AP) has 26 citations. These appear across journals including Bioactive Materials, Phytomedicine, Journal of Translational Medicine, Apoptosis, Antioxidants, Journal of Ethnopharmacology, European Journal of Pharmacology, Mitochondrion, Chemical & Biological Interactions, FEBS Journal, Molecular Immunology, Kidney Disease, Journal of Diabetes Research, Experimental Cell Research, Fitoterapia, Journal of Nephrology, International Urology and Nephrology, and Lupus. Research fields center on nephrology: diabetic nephropathy, podocyte injury, renal fibrosis, nephrotic syndrome, and kidney disease mechanisms.

Automatically generated from the 26 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat,human	IF	Bioact Mater; Bioengineered platelet nanoplatform enables renal-targeted dexamethasone delivery for chronic nephritis therapy with dual anti-inflammatory/anti-fibrotic effects and minimized systemic toxicity; Authors - Lingling Zhang
rat,mouse	WB	Phytomedicine; Fangji Huangqi Tang alleviated chronic kidney disease by regulating intestinal bacteria to inhibit the AHR/ROS pathway.; Authors - Aiping Li
mouse	IF	J Transl Med; Gut microbiota dysbiosis contributes to diabetic nephropathy via affecting renal lipid deposition and inflammatory responses.; Authors - Ping Liu
mouse	WB,IF	Apoptosis; Inhibition of SIRT1/HSF1 pathway contributes to doxorubicin-induced nephrotoxicity in ovarian tumor-bearing mice; Authors - Mo Chen
mouse	WB	Antioxidants (Basel); Identification and Validation of the miR/RAS/RUNX2 Autophagy Regulatory Network in AngII-Induced Hypertensive Nephropathy in MPC5 Cells Treated with Hydrogen Sulfide Donors; Authors - Qing Ye
rat	IF	J Ethnopharmacol; Supplemented Gegen Qinlian Decoction Formula attenuates podocyte mitochondrial fission and renal fibrosis in diabetic kidney disease by inhibiting TNF-α-mediated necroptosis, compared with empagliflozin; Authors - Yu Wang