NPL Polyclonal antibody

NPL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16715-1-AP

Synonyms

N-acetylneuraminate pyruvate-lyase, N-acetylneuraminate lyase, N acetylneuraminate lyase, EC:4.1.3.3, C1orf13

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Validation Data Gallery

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  WB analysis using 16715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 16715-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16715-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16715-1-AP (NPL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 16715-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16715-1-AP (NPL antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 16715-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NPL antibody (16715-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse kidney tissue, Raji cells, rat brain tissue, rat kidney tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16715-1-AP targets NPL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPL fusion protein Ag10137
• Full Name: N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)
• Calculated Molecular Weight: 27 kDa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC058003
• Gene Symbol: NPL
• Gene ID (NCBI): 80896
• RRID: AB_2154866
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The N-acetylneuraminate lyase(NPL) from human (hNAL) which consists of 320 amino acids, has a molecular mass of 35.2 kDa and it catalyses the reversible aldol reaction of sialic acid, and has been extensively used in the synthesis of sialic acids and its analogues(PMID:19057931). The enzyme is widely distributed and are present in numerous pro- and eukaryotic cells, in which they are localized only in the cytosol(PMID:12453637). It belongs to the DHDPS family and has 5 isoforms produced by alternative splicing.