Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse heart tissue, rat heart tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11638-1-AP targets NPLOC4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2219 Product name: Recombinant human NPLOC4 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 261-608 aa of BC025930 Sequence: DIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNMCRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKRSEQWATIEQLCSTVGGQLPGLHEYGAVGGSTHTATAAMWACQHCTFMNQPGTGHCEMCSLPRT Predict reactive species |
| Full Name | nuclear protein localization 4 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 608 aa, 68 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC025930 |
| Gene Symbol | NPLOC4 |
| Gene ID (NCBI) | 55666 |
| RRID | AB_10597107 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TAT6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NPLOC4 antibody 11638-1-AP | Download protocol |
| IP protocol for NPLOC4 antibody 11638-1-AP | Download protocol |
| WB protocol for NPLOC4 antibody 11638-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NPLOC4 Polyclonal antibody (catalog no. 11638-1-AP) has 20 citations. Journals include Science, Sci Signal, J Biol Chem, J Cell Sci, Mol Cell Biol, FASEB J, Cell Signal, Mol Cell Proteomics, Mater Today Bio, Bioorg Chem, Biochim Biophys Acta, Steroids, Virus Res, Mol Biol Rep, J Biochem Mol Toxicol, mBio, Open Biol, and bioRxiv. Research fields span ubiquitination, stress granules, ERAD, autophagy, VCP/p97 signaling, and cancer (prostate, bladder, pancreatic, hepatocellular), with applications mainly in WB, IF, and IP.
| Species | Application | Title |
|---|---|---|
Science Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner. | ||
Mater Today Bio A thermosensitive hydrogel with synergistic stromal targeting and antitumor immunity modulation for pancreatic cancer immunotherapy | ||
Sci Signal TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing | ||
Mol Cell Proteomics Localized K63 ubiquitin signaling is regulated by VCP/p97 during oxidative stress | ||
Bioorg Chem ATI-1 mediated disruption of the VCP-UFL1-Beclin1 axis thwarts autophagy initiation to trigger metabolic catastrophe in autophagy-addicted cancers. | ||
Biochim Biophys Acta Mol Basis Dis Trim21 modulates endoplasmic reticulum-associated degradation and sensitizes cancer cells to ER stress-induced apoptosis by inhibiting VCP/Npl4/UFD1 assembly |
