NPLOC4 Polyclonal antibody

• Featured Product
• KD/KO Validated

NPLOC4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 11638-1-AP

Synonyms

KIAA1499, NPLOC4, Protein NPL4

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Validation Data Gallery

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  WB analysis of mouse kidney using 11638-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11638-1-AP (NPLOC4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11638-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11638-1-AP (NPLOC4 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse heart using 11638-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11638-1-AP (NPLOC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 11638-1-AP

  IP result of anti-NPLOC4 (IP:11638-1-AP, 4ug; Detection:11638-1-AP 1:600) with mouse kidney tissue lysate 6000ug.

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  IHC staining of human kidney using 11638-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11638-1-AP (NPLOC4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11638-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11638-1-AP (NPLOC4 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse heart tissue, rat heart tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11638-1-AP targets NPLOC4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPLOC4 fusion protein Ag2219
• Full Name: nuclear protein localization 4 homolog (S. cerevisiae)
• Calculated Molecular Weight: 608 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC025930
• Gene Symbol: NPLOC4
• Gene ID (NCBI): 55666
• RRID: AB_10597107
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IP	Science; Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.; Authors - Youngdae Gwon
mouse	WB	Sci Signal; TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing; Authors - Liangbin Lin
human	WB,IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli ; KD Validated
human	WB	J Biol Chem; Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.; Authors - Tao Wang
human	WB	Mol Cell Biol; The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.; Authors - Rakesh Ganji ; KD Validated
human	WB	Virus Res; Involvement of VCP/UFD1/Nucleolin in the viral entry of Enterovirus A species.; Authors - Jingjing Yan ; KD Validated