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NPPA Polyclonal antibody, PBS Only (27426-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 27426-1-AP (same clone as 27426-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.
WB analysis using 27426-1-AP (same clone as 27426-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 27426-1-AP (same clone as 27426-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 27426-1-AP (same clone as 27426-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.
IF Staining of C2C12 using 27426-1-AP (same clone as 27426-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using NPPA antibody (27426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 27426-1-AP (same clone as 27426-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NPPA antibody (27426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

NPPA Polyclonal antibody, PBS Only

Name: NPPA Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 27426-1-PBS

NPPA Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 27426-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ANP, PND, ANF, ATFB6, Atrial natriuretic factor

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

27426-1-PBS targets NPPA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26692 Product name: Recombinant human NPPA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-153 aa of BC005893 Sequence: NPMYNAVSNADLMDFKNLLDHLEEKMPLEDEVVPPQVLSEPNEEAGAALSPLPEVPPWTGEVSPAQRDGGALGRGPWDSSDRSALLKSKLRALLTAPRSLRRSSCFGGRMDRIGAQSGLGCNSFRYRR Predict reactive species
Full Name	natriuretic peptide precursor A
Calculated Molecular Weight	153 aa, 17 kDa
Observed Molecular Weight	19 kDa
GenBank Accession Number	BC005893
Gene Symbol	NPPA
Gene ID (NCBI)	4878
RRID	AB_2880868
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P01160
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Natriuretic peptide precursor-A (NPPA) is an early and specific marker for functional myocardium of the embryonic heart. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. NPPA is expressed primarily in the heart, where the expression level is higher in atria than ventricles. Low levels of ANP expression have been detected in other tissues, including the lung, aorta, brain, adrenal gland, kidney and uterus.