NPRL3 Polyclonal antibody, PBS Only

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• NPRL3 Polyclonal antibody, PBS Only (26446-1-PBS)

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  WB analysis using 26446-1-AP (same clone as 26446-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26446-1-AP (C16orf35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26446-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 26446-1-AP (same clone as 26446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26446-1-AP (NPRL3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26446-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 26446-1-AP (same clone as 26446-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using NPRL3 antibody (26446-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26446-1-PBS in a different storage buffer formulation.

NPRL3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 26446-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C16orf35, 14 gene protein, -14 gene protein, Alpha-globin regulatory element-containing gene protein, CGTHBA

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Product Information

26446-1-PBS targets NPRL3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24045

  Product name: Recombinant human C16orf35 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-390 aa of BC004185

  Sequence: MADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAASSLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKTTSAVKNLQQLAQDADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPSHDLPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEEPRPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQRMTENLLASLSEHERAAILSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQLLMLFDKFRSVLVVTTHEDPVIAVFQALLP

• Full Name: chromosome 16 open reading frame 35
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC004185
• Gene Symbol: C16orf35
• Gene ID (NCBI): 8131
• RRID: AB_3742190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12980
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NPRL3 (Nitrogen Permease Regulator Like 3) is a highly conserved tumor suppressor protein. As a core component of the GATOR1 complex, it serves as a key node in the amino acid sensing pathway and negatively regulates the mTORC1 signaling pathway, thereby controlling cell growth, proliferation, and autophagy. Loss of its function leads to hyperactivation of mTORC1 signaling. In neuroscience, loss-of-function mutations in NPRL3 are an important genetic cause of familial focal epilepsy, such as focal epilepsy with variable foci. In oncology, it is frequently inactivated through epigenetic silencing in various cancers, making it a significant tumor suppressor and a potential biomarker.