NPVF Polyclonal antibody

NPVF Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 22311-1-AP

Synonyms

C7orf9, FMRFamide related peptides, Neuropeptide RFRP 3, Neuropeptide VF, neuropeptide VF precursor, NPSF, NPVF, RFRP

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Validation Data Gallery

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  IHC staining of human kidney using 22311-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22311-1-AP (NPSF antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 22311-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22311-1-AP (NPSF antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human hypothalamus using 22311-1-AP

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 22311-1-AP (NPSF antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human hypothalamus using 22311-1-AP

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 22311-1-AP (NPSF antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human kidney tissue, human hypothalamus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22311-1-AP targets NPVF in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: neuropeptide VF precursor
• Calculated Molecular Weight: 22 kDa
• GenBank Accession Number: NM_022150
• Gene Symbol: NPVF
• Gene ID (NCBI): 64111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human RFamide-related peptide gene, RFRP (also designated NPVF or C7orf9), is responsible for encoding three small neuropeptides designated NPSF, RFRP-1 RFRP-2 and RFRP-3 (NPVF). Neuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP, but RFRP-2 shows no inhibitory activity. Neuropeptide RFRP-1 induces secretion of prolactin in rats. Neuropeptide NPVF blocks morphine-induced analgesia. This antibody can recognize neuropeptide VF precursor and neuropeptide NPSF.