NPTX2 Polyclonal antibody, PBS Only

• 

  WB analysis using 10889-1-AP (same clone as 10889-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 10889-1-AP (same clone as 10889-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 10889-1-AP (same clone as 10889-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 10889-1-AP (same clone as 10889-1-PBS)

  IP result of anti-NPTX2 (IP:10889-1-AP, 4ug; Detection:10889-1-AP 1:600) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 10889-1-AP (same clone as 10889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 10889-1-AP (same clone as 10889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 10889-1-AP (same clone as 10889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10889-1-AP (NPTX2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 10889-1-AP (same clone as 10889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 10889-1-AP (same clone as 10889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 10889-1-AP (same clone as 10889-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NPTX2 antibody (10889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NPTX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10889-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

---

Product Information

10889-1-PBS targets NPTX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPTX2 fusion protein Ag1326
• Full Name: neuronal pentraxin II
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC009924
• Gene Symbol: NPTX2
• Gene ID (NCBI): 4885
• RRID: AB_2153875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P47972
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)