Glucocorticoid receptor Polyclonal antibody

• Featured Product
• KD/KO Validated

Glucocorticoid receptor Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

74

Reviews (4)

Cat no : 24050-1-AP

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

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Validation Data Gallery

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  WB analysis of HepG2 using 24050-1-AP

  WB result of Glucocorticoid receptor antibody (24050-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glucocorticoid receptor transfected HepG2 cells.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 24050-1-AP

  IP result of anti-Glucocorticoid receptor(IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:4000) with HepG2 cells lysate 1720 ug.

• 

  IP experiment of HepG2 using 24050-1-AP

  IP result of anti-Glucocorticoid receptor(IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:4000) with HepG2 cells lysate 1720 ug.

• 

  IP experiment of mouse heart using 24050-1-AP

  IP Result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:500) with mouse heart tissue lysate 4400ug.

• 

  IHC staining of human prostate cancer using 24050-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 24050-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 24050-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 24050-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 24050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 24050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, DU 145 cells, mouse brain tissue, rat brain tissue, PC-12 cells
• Positive IP detected in: HepG2 cells, mouse heart tissue
• Positive IHC detected in: human prostate cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 11 publications below
• WB: See 56 publications below
• IHC: See 3 publications below
• IF: See 14 publications below
• IP: See 2 publications below
• CoIP: See 5 publications below
• ChIP: See 15 publications below

Product Information

24050-1-AP targets Glucocorticoid receptor in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Rare minnow
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Glucocorticoid receptor fusion protein Ag21146
• Full Name: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• Calculated molecular weight: 86 kDa
• Observed molecular weight: 94-97 kDa
• GenBank accession number: BC015610
• Gene symbol: NR3C1
• Gene ID (NCBI): 2908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].

Publications

Species	Application	Title
human	WB	Cell Metab; Individual-specific functional epigenomics reveals genetic determinants of adverse metabolic effects of glucocorticoids.; Authors - Wenxiang Hu
mouse	IHC	Cell Metab; A macrophage-hepatocyte glucocorticoid receptor axis coordinates fasting ketogenesis.; Authors - Anne Loft
mouse	chIP	Mol Cell; Cistromic Reprogramming of the Diurnal Glucocorticoid Hormone Response by High-Fat Diet.; Authors - Fabiana Quagliarini
mouse	WB,ChIP,IP	J Clin Invest; REVERBa couples the circadian clock to hepatic glucocorticoid action.; Authors - Giorgio Caratti
mouse	ChIP	Mol Psychiatry; The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice.; Authors - Alexander S Häusl
rat	ChIP	Nat Commun; Distinct regulation of hippocampal neuroplasticity and ciliary genes by corticosteroid receptors.; Authors - Karen R Mifsud

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ping (Verified Customer) (06-04-2019)

This antibody works well for immunoprecipitation and western blot.

• Applications: Western Blot, Immunoprecipitation,
• Cell Tissue Type: mouse brain tissue

FH

Yu Siong (Verified Customer) (04-23-2019)

Multiple bands observed - Total of 5 bands, not suitable for porcine brain endothelial cells, as non-specific binding tend to occur.Consistent in two Independent experiments

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Porcine brain endothelial cells

FH

Praveen (Verified Customer) (02-25-2019)

WORKS VERY WELL.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Cell culture, ChIP,
• Cell Tissue Type: Ac16

FH

Shubham (Verified Customer) (01-31-2019)

Antibody works great.

• Applications: Western Blot, Immunohistochemistry, Cell culture,
• Primary Antibody Dilution: 1:10
• Cell Tissue Type: Ac16

Glucocorticoid receptor Antibody Western Blot,Immunohistochemistry,Cell culture, validation (1:10 dilution) in Ac16 (Cat no:24050-1-AP)

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