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Glucocorticoid receptor Polyclonal antibody
Glucocorticoid receptor Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, IP, IHC, IF, CoIP, ChIP, ELISA
Cat no : 24050-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, HepG2 cells, DU 145 cells, mouse brain tissue, rat brain tissue, PC-12 cells|
|Positive IP detected in||HepG2 cells, mouse heart tissue|
|Positive IHC detected in||human prostate cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 11 publications below|
|WB||See 56 publications below|
|IHC||See 3 publications below|
|IF||See 14 publications below|
|IP||See 2 publications below|
|CoIP||See 5 publications below|
|ChIP||See 15 publications below|
24050-1-AP targets Glucocorticoid receptor in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Rare minnow|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Glucocorticoid receptor fusion protein Ag21146|
|Full Name||nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)|
|Calculated molecular weight||86 kDa|
|Observed molecular weight||94-97 kDa|
|GenBank accession number||BC015610|
|Gene ID (NCBI)||2908|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].
|Product Specific Protocols|
|WB protocol for Glucocorticoid receptor antibody 24050-1-AP||Download protocol|
|IHC protocol for Glucocorticoid receptor antibody 24050-1-AP||Download protocol|
|IF protocol for Glucocorticoid receptor antibody 24050-1-AP||Download protocol|
|IP protocol for Glucocorticoid receptor antibody 24050-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Individual-specific functional epigenomics reveals genetic determinants of adverse metabolic effects of glucocorticoids.
A macrophage-hepatocyte glucocorticoid receptor axis coordinates fasting ketogenesis.
Cistromic Reprogramming of the Diurnal Glucocorticoid Hormone Response by High-Fat Diet.
J Clin Invest
REVERBa couples the circadian clock to hepatic glucocorticoid action.
The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice.
Distinct regulation of hippocampal neuroplasticity and ciliary genes by corticosteroid receptors.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ping (Verified Customer) (06-04-2019)
This antibody works well for immunoprecipitation and western blot.
Yu Siong (Verified Customer) (04-23-2019)
Multiple bands observed - Total of 5 bands, not suitable for porcine brain endothelial cells, as non-specific binding tend to occur.Consistent in two Independent experiments
Praveen (Verified Customer) (02-25-2019)
WORKS VERY WELL.
Shubham (Verified Customer) (01-31-2019)
Antibody works great.