Glucocorticoid receptor Polyclonal antibody

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  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 24050-1-AP

  IP result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:500) with mouse heart tissue lysate 4400ug.

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  IP experiment of HepG2 using 24050-1-AP

  IP result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:4000) with HepG2 cells lysate 1720 ug.

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  IP experiment of HepG2 using 24050-1-AP

  IP result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:4000) with HepG2 cells lysate 1720 ug.

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  IHC staining of human prostate cancer using 24050-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 24050-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 24050-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 24050-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 24050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A549 using 24050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 24050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

Glucocorticoid receptor Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24050-1-AP

Publications(103)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

NR3C1, GRL, GR, GCR, GCCR

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, DU 145 cells, mouse brain tissue, rat brain tissue, PC-12 cells
• Positive IP detected in: HepG2 cells, mouse heart tissue
• Positive IHC detected in: human prostate cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 73 publications below
• IHC: See 9 publications below
• IF: See 17 publications below
• IP: See 3 publications below
• CoIP: See 5 publications below
• ChIP: See 20 publications below

Product Information

24050-1-AP targets Glucocorticoid receptor in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, zebrafish, rare minnow
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Glucocorticoid receptor fusion protein Ag21146
• Full Name: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 94-97 kDa
• GenBank Accession Number: BC015610
• Gene Symbol: Glucocorticoid receptor
• Gene ID (NCBI): 2908
• RRID: AB_2813890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04150
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

Publications

Species	Application	Title
human	WB	Cell Metab; Individual-specific functional epigenomics reveals genetic determinants of adverse metabolic effects of glucocorticoids.; Authors - Wenxiang Hu
mouse	IHC	Cell Metab; A macrophage-hepatocyte glucocorticoid receptor axis coordinates fasting ketogenesis.; Authors - Anne Loft
human	WB	Sci Adv; Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.; Authors - Leiming Wang
mouse	chIP	Mol Cell; Cistromic Reprogramming of the Diurnal Glucocorticoid Hormone Response by High-Fat Diet.; Authors - Fabiana Quagliarini
mouse	WB,ChIP,IP	J Clin Invest; REVERBa couples the circadian clock to hepatic glucocorticoid action.; Authors - Giorgio Caratti
mouse	ChIP	Mol Psychiatry; The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice.; Authors - Alexander S Häusl

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ping (Verified Customer) (06-04-2019)

This antibody works well for immunoprecipitation and western blot.

• Applications: Western Blot, Immunoprecipitation,
• Cell Tissue Type: mouse brain tissue

FH

Yu Siong (Verified Customer) (04-23-2019)

Multiple bands observed - Total of 5 bands, not suitable for porcine brain endothelial cells, as non-specific binding tend to occur.Consistent in two Independent experiments

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Porcine brain endothelial cells

FH

Praveen (Verified Customer) (02-25-2019)

WORKS VERY WELL.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Cell culture, ChIP,
• Cell Tissue Type: Ac16

FH

Shubham (Verified Customer) (01-31-2019)

Antibody works great.

• Applications: Western Blot, Immunohistochemistry, Cell culture,
• Primary Antibody Dilution: 1:10
• Cell Tissue Type: Ac16

Glucocorticoid receptor Antibody Western Blot,Immunohistochemistry,Cell culture, validation (1:10 dilution) in Ac16 (Cat no:24050-1-AP)