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Glucocorticoid receptor Polyclonal antibody
Glucocorticoid receptor Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 24050-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, DU 145 cells, mouse brain tissue, rat brain tissue, PC-12 cells |
Positive IP detected in | HepG2 cells, mouse heart tissue |
Positive IHC detected in | human prostate cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:8000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 11 publications below |
WB | See 56 publications below |
IHC | See 3 publications below |
IF | See 14 publications below |
IP | See 2 publications below |
CoIP | See 5 publications below |
ChIP | See 15 publications below |
Product Information
24050-1-AP targets Glucocorticoid receptor in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, Rare minnow |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Glucocorticoid receptor fusion protein Ag21146 |
Full Name | nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) |
Calculated molecular weight | 86 kDa |
Observed molecular weight | 94-97 kDa |
GenBank accession number | BC015610 |
Gene symbol | NR3C1 |
Gene ID (NCBI) | 2908 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].
Protocols
Product Specific Protocols | |
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WB protocol for Glucocorticoid receptor antibody 24050-1-AP | Download protocol |
IHC protocol for Glucocorticoid receptor antibody 24050-1-AP | Download protocol |
IF protocol for Glucocorticoid receptor antibody 24050-1-AP | Download protocol |
IP protocol for Glucocorticoid receptor antibody 24050-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab Individual-specific functional epigenomics reveals genetic determinants of adverse metabolic effects of glucocorticoids. | ||
Cell Metab A macrophage-hepatocyte glucocorticoid receptor axis coordinates fasting ketogenesis. | ||
Mol Cell Cistromic Reprogramming of the Diurnal Glucocorticoid Hormone Response by High-Fat Diet. | ||
Mol Psychiatry The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice. | ||
Nat Commun Distinct regulation of hippocampal neuroplasticity and ciliary genes by corticosteroid receptors. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ping (Verified Customer) (06-04-2019) | This antibody works well for immunoprecipitation and western blot.
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FH Yu Siong (Verified Customer) (04-23-2019) | Multiple bands observed - Total of 5 bands, not suitable for porcine brain endothelial cells, as non-specific binding tend to occur.Consistent in two Independent experiments
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FH Praveen (Verified Customer) (02-25-2019) | WORKS VERY WELL.
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FH Shubham (Verified Customer) (01-31-2019) | Antibody works great.
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