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Glucocorticoid receptor Polyclonal antibody
Glucocorticoid receptor Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 24050-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, HepG2 cells, DU 145 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human prostate cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24050-1-AP is Glucocorticoid receptor Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Rare minnow|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Glucocorticoid receptor fusion protein Ag21146|
|Full Name||nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)|
|Calculated molecular weight||86 kDa|
|Observed molecular weight||94-97 kDa|
|GenBank accession number||BC015610|
|Gene ID (NCBI)||2908|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].
|Product Specific Protocols|
|WB protocol for Glucocorticoid receptor antibody 24050-1-AP||Download protocol|
|IHC protocol for Glucocorticoid receptor antibody 24050-1-AP||Download protocol|
|IF protocol for Glucocorticoid receptor antibody 24050-1-AP||Download protocol|
|IP protocol for Glucocorticoid receptor antibody 24050-1-AP||Download protocol|
|Click here to view our Standard Protocols|
E47 modulates hepatic glucocorticoid action.
J Mol Endocrinol
An improved method for quantitative ChIP studies of nuclear receptor function.
Cortisol Excess-Mediated Mitochondrial Damage Induced Hippocampal Neuronal Apoptosis in Mice Following Cold Exposure.
Corticosterone Excess-Mediated Mitochondrial Damage Induces Hippocampal Neuronal Autophagy in Mice Following Cold Exposure.
Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.
α-Viniferin activates autophagic apoptosis and cell death by reducing glucocorticoid receptor expression in castration-resistant prostate cancer cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ping (Verified Customer) (06-04-2019)
This antibody works well for immunoprecipitation and western blot.
Yu Siong (Verified Customer) (04-23-2019)
Multiple bands observed - Total of 5 bands, not suitable for porcine brain endothelial cells, as non-specific binding tend to occur.Consistent in two Independent experiments
Praveen (Verified Customer) (02-25-2019)
WORKS VERY WELL.
Shubham (Verified Customer) (01-31-2019)
Antibody works great.