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NR3C2 Polyclonal antibody, PBS Only (21854-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)
WB result of NR3C2 antibody (21854-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR3C2 transfected HEK-293 cells. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
WB analysis using 21854-1-AP (same clone as 21854-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)
IP result of anti-NR3C2 (IP:21854-1-AP, 4ug; Detection:21854-1-AP 1:500) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 21854-1-AP (same clone as 21854-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 21854-1-AP (same clone as 21854-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 21854-1-AP (same clone as 21854-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 21854-1-AP (same clone as 21854-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 21854-1-AP (same clone as 21854-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

NR3C2 Polyclonal antibody, PBS Only

Name: NR3C2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 21854-1-PBS

NR3C2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 21854-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

MCR, MLR, MR

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

21854-1-PBS targets NR3C2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16410 Product name: Recombinant human NR3C2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC111758 Sequence: METKGYHSLPEGLDMERRWGQVSQAVERSSLGPTERTDENNYMEIVNVSCVSGAIPNNSTQGSSKEKQELLPCLQQDNNRPGILTSDIKTELESKELSATVAESMGLYMDSVRDADYSYEQQNQQGSMSPAKIYQNVEQLVKFYKGNGHRPSTLSCVNTPLRSFMSDSGSSVNGGVMRAIVKSPIMCHEKSPSVCSPLNMTSSVCSPAGINSVSSTTASFGSFPVHSPITQGTPLTCSPNVENRGSRSHSPAHASNVGSPLSSPLSSMKSSISSPPSHCSVKSPVSSPNNVTLRSSVSSPANINNSRCSVSSPSNTNNRSTLSSPAASTVGSICSPVNNAFSYTASGTSA Predict reactive species
Full Name	nuclear receptor subfamily 3, group C, member 2
Calculated Molecular Weight	984 aa, 107 kDa
Observed Molecular Weight	94-110 kDa
GenBank Accession Number	BC111758
Gene Symbol	NR3C2
Gene ID (NCBI)	4306
RRID	AB_10858785
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08235
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.