Tested Applications
| Positive WB detected in | MG132 treated HCT 116 cells, MG132 treated NIH/3T3 cells, MG132 treated HepG2 cells |
| Positive IF/ICC detected in | MG132 treated NIH/3T3 cells, MG132 treated HepG2 cells |
It is recommended to use stimulated samples.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66504-1-Ig targets NRF2/NFE2L2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, zebrafish |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9469 Product name: Recombinant human NRF2, NFE2L2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 256-605 aa of BC011558 Sequence: SILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMPSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDAQCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLLKEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDVKKN Predict reactive species |
| Full Name | nuclear factor (erythroid-derived 2)-like 2 |
| Calculated Molecular Weight | 605 aa, 68 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC011558 |
| Gene Symbol | NRF2 |
| Gene ID (NCBI) | 4780 |
| RRID | AB_2881867 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16236 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NRF2/NFE2L2 antibody 66504-1-Ig | Download protocol |
| WB protocol for NRF2/NFE2L2 antibody 66504-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Nrf2 antibody (Cat# 66504-1-Ig) has 218 total citations published in journals including Nature Communications, Cell Host & Microbe, Redox Biology, Journal of Clinical Investigation, Advanced Science, Free Radical Biology and Medicine, Antioxidants, Phytomedicine, and International Journal of Molecular Sciences. Associated research fields include oxidative stress and antioxidant signaling, ferroptosis, neurodegeneration, hepatoprotection, oncology, cardiovascular disease, renal injury, inflammation, gut microbiota modulation, and food/nutrition science.
| Species | Application | Title |
|---|---|---|
Bioact Mater Manganese oxide nanozyme-loaded CO-releasing halloysite nanotubes for remodeling colonic immune, microbiota, and intestinal barrier homeostasis with concurrent pain relief in ulcerative colitis. | ||
Cell Host Microbe Gut microbiota-derived ergothioneine alleviates antipsychotic-induced synaptic and cognitive impairments. | ||
Nat Commun Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection. | ||
Redox Biol Phactr4 promotes oxidative stress and behavioral disorder caused by chronic stress via regulating PP1/GSK3-β pathway. | ||
Redox Biol Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer. | ||
Redox Biol Ursodeoxycholic acid mitigates cerebral ischemia/reperfusion injury by inhibiting thrombin-induced lipid peroxidation through activation of ALDH3A1. |
Reviews
What customers say
Both reviewers gave 5-star ratings for Western Blot applications using a 1:1000 dilution. One user reported two specific bands with no background. The other noted that while tissue samples showed bands at varying positions, cancer cell lines produced significantly clearer results, confirming 1:1000 as the optimal dilution. Overall, users are satisfied with the antibody's specificity and performance in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shunbin (Verified Customer) (05-14-2024) | Two specific bands without background. Great!
 |
Shinford (Verified Customer) (10-26-2023) | In tissues, we observed bands at varying positions, but in cancer cell lines, the bands appeared significantly clearer. The optimal concentration was found to be 1:1000.
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