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NRF2, NFE2L2 Antibody 4 Publications

Mouse Monoclonal| Catalog number: 66504-1-Ig |CloneNo.: 1E9E3

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Tested Applications

Applications:
WB, ELISA
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NRF2, NFE2L2 Antibody WB HeLa cells 66504-1-Ig

Untreated and MG132 treated Hela cells were subjected to SDS PAGE followed by western blot with 66504-1-Ig (NRF2, NFE2L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Species specificity:
Human, mouse
Positive WB detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:4000
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
NRF2, NFE2L2 fusion protein Ag9469
Full name:
nuclear factor (erythroid-derived 2)-like 2
Calculated molecular weight:
605aa,68 kDa
Observed molecular weight:
110 kDa
GenBank accession number:
BC011558
Gene ID (NCBI):
4780
Gene symbol
NFE2L2
Synonyms
HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2

Background

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. The calculated molecular weight of Nrf2 protein is about 67 kDa, but Nrf2 protein can form a heterodimer with MafG in cell nucleus and molecular weight of heterodimer is 110 kDa.


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