NRF2, NFE2L2 Monoclonal antibody

• Featured Product
• KD/KO Validated

NRF2, NFE2L2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (4)

Applications

WB, IF, CoIP,ELISA

Conjugate

Unconjugated

Publications

18

CloneNo.

1E9E3

Cat no : 66504-1-Ig

Synonyms

HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2

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Validation Data Gallery

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  WB analysis of HeLa using 66504-1-Ig

  Untreated and MG132 treated Hela cells were subjected to SDS PAGE followed by western blot with 66504-1-Ig (NRF2, NFE2L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 17 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

66504-1-Ig targets NRF2, NFE2L2 in WB, IF, CoIP,ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NRF2, NFE2L2 fusion protein Ag9469
• Full Name: nuclear factor (erythroid-derived 2)-like 2
• Calculated molecular weight: 605 aa, 68 kDa
• Observed molecular weight: 110 kDa
• GenBank accession number: BC011558
• Gene symbol: NFE2L2
• Gene ID (NCBI): 4780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the biologically relevant molecular weight of Nrf2 is ~95-110 kDa and not the predicted ~55-65 kDa based on its 2-kilobase open reading frame. The data discussed and presented here will hopefully lead to a uniform acceptance that future experiments and publications should be designed around detecting Nrf2 at the correct molecular weight of ~95-120 kDa (PMID: 22703241).

Publications

Species	Application	Title
mouse	WB	J Cell Mol Med; Aucubin inhibited lipid accumulation and oxidative stress via Nrf2/HO-1 and AMPK signalling pathways.; Authors - Bingyu Shen
rat	WB	Biomed Res Int; Effect of Quercetin on PC12 Alzheimer's Disease Cell Model Induced by Aβ25-35 and Its Mechanism Based on Sirtuin1/Nrf2/HO-1 Pathway.; Authors - Xinjun Yu
rat	WB	Med Sci Monit; Protective Effects of Ischemic Postconditioning on Livers in Rats with Limb Ischemia-Reperfusion via Glycogen Synthase Kinase 3 beta (GSK-3β)/Fyn/Nuclear Receptor-Erythroid-2-Related Factor (Nrf2) Pathway.; Authors - Qibing Niu
human	WB	Oxid Med Cell Longev; Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK.; Authors - Ning Ma ; KD Validated
mouse	WB	Med Sci Monit; Activation of the Nuclear Erythroid 2-Related Factor 2 Antioxidant Responsive Element (Nrf2-ARE) Signaling Pathway Alleviates Acute Graft-Versus-Host Disease by Reducing Oxidative Stress and Inhibiting Infiltration of Inflammatory Cells in an Allogeneic Stem Cell Transplantation Mouse Model.; Authors - Ting Yi
mouse	WB	Respir Physiol Neurobiol; Bakuchiol ameliorates cerebral ischemia-reperfusion injury by modulating NLRP3 inflammasome and Nrf2 signaling.; Authors - Yuewei Xu