NRF2, NFE2L2 Monoclonal antibody

• Featured Product
• KD/KO Validated

NRF2, NFE2L2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (4)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

114

CloneNo.

1E9E3

Reviews (2)

Cat no : 66504-1-Ig

Synonyms

HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2, NRF2, NFE2L2

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Validation Data Gallery

• 

  WB analysis of HeLa using 66504-1-Ig

  Untreated and MG132 treated Hela cells were subjected to SDS PAGE followed by western blot with 66504-1-Ig (NRF2, NFE2L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 14 publications below
• WB: See 105 publications below
• IHC: See 8 publications below
• IF: See 22 publications below
• IP: See 1 publications below
• CoIP: See 4 publications below
• ChIP: See 1 publications below

Product Information

66504-1-Ig targets NRF2, NFE2L2 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NRF2, NFE2L2 fusion protein Ag9469
• Full Name: nuclear factor (erythroid-derived 2)-like 2
• Calculated Molecular Weight: 605 aa, 68 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC011558
• Gene Symbol: NRF2
• Gene ID (NCBI): 4780
• RRID: AB_2881867
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Publications

Species	Application	Title
human	WB,IHC,IP	J Clin Invest; FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway; Authors - Yunjiang Zhou
mouse	CoIP	Nat Commun; Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection.; Authors - Yao Wei
mouse	WB	Aging Cell; Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis.; Authors - Yongxin Wu
human	WB	Redox Biol; Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.; Authors - Shumin Ouyang
mouse,human	WB,IF	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin
human	WB,IHC,IF	Phytomedicine; Tiliroside targets TBK1 to induce ferroptosis and sensitize hepatocellular carcinoma to sorafenib; Authors - Chen Yang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shunbin (Verified Customer) (05-14-2024)

Two specific bands without background. Great!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

NRF2, NFE2L2 Antibody Western Blot validation (1:1000 dilution) in (Cat no:66504-1-Ig)

FH

Shinford (Verified Customer) (10-26-2023)

In tissues, we observed bands at varying positions, but in cancer cell lines, the bands appeared significantly clearer. The optimal concentration was found to be 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer cell lines

NRF2, NFE2L2 Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines (Cat no:66504-1-Ig)