NRG1, isoform SMDF Monoclonal antibody

NRG1, isoform SMDF Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse, rat, rabbit

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2C6D12

Cat no : 66492-1-Ig

Synonyms

ARIA, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, NDF, Neu differentiation factor, neuregulin 1, NRG1, Pro NRG1, SMDF

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Validation Data Gallery

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  WB analysis using 66492-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of pig cerebellum using 66492-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 66492-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 66492-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66492-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66492-1-Ig (NRG1, isoform SMDF antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 66492-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform SMDF antibody (66492-1-Ig, Clone: 2C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat brain tissue, HepG2 cells, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue, mouse brain tissue, rabbit brain tissue
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

66492-1-Ig targets NRG1, isoform SMDF in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, mouse, rat, rabbit samples.

• Tested Reactivity: Human, pig, mouse, rat, rabbit
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NRG1, isoform SMDF fusion protein Ag0803
• Full Name: neuregulin 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC007675
• Gene Symbol: NRG1
• Gene ID (NCBI): 3084
• RRID: AB_2881857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The antibody is specific to NRG1 isoform SMDF.

Publications

Species	Application	Title
mouse	WB,IF	Prog Neuropsychopharmacol Biol Psychiatry; The Notch1/Hes1 pathway regulates Neuregulin 1/ErbB4 and participates in microglial activation in rats with VPA-induced autism; Authors - Yanan Deng
rat	IHC	J Cell Mol Med; PMID: 32073745; Authors - Jianxia Wen
mouse	IF	Int J Mol Sci; Dysregulation of ErbB4 Signaling Pathway in the Dorsal Hippocampus after Neonatal Hypoxia-Ischemia and Late Deficits in PV+ Interneurons, Synaptic Plasticity and Working Memory; Authors - Harisa Spahic