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- KD/KO Validated
NSDHL Polyclonal antibody
NSDHL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 15111-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, human heart tissue, mouse brain tissue |
| Positive IHC detected in | human skin cancer tissue, human cervical cancer tissue, human colon tissue, human colon cancer tissue, human lung tissue, human lung cancer tissue, human ovary tissue, human ovary tumor tissue, human stomach tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3200 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 7 publications below |
Product Information
15111-1-AP targets NSDHL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NSDHL fusion protein Ag7097 |
| Full Name | NAD(P) dependent steroid dehydrogenase-like |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa, 40 kDa |
| GenBank Accession Number | BC000245 |
| Gene Symbol | NSDHL |
| Gene ID (NCBI) | 50814 |
| RRID | AB_2155681 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NSDHL(Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating) encodes a sterol dehydrogenase or decarboxylase involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis. Mutations in this gene are associated with human CHILD syndrome (congenital hemidysplasia with ichthyosiform nevus and limb defects), an X-linked, male lethal disorder, as well as the mouse mutations bare patches and striated.(PMID: 14506130).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NSDHL antibody 15111-1-AP | Download protocol |
| IHC protocol for NSDHL antibody 15111-1-AP | Download protocol |
| IF protocol for NSDHL antibody 15111-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Cholesterol Pathway Inhibition Induces TGF-β Signaling to Promote Basal Differentiation in Pancreatic Cancer.
| ||
Cancer Discov Targeting C4-demethylating genes in the cholesterol pathway sensitizes cancer cells to EGF receptor inhibitors via increased EGF receptor degradation. | ||
Clin Cancer Res Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma.
| ||
Cell Rep Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR. | ||
Cell Cycle Insm2 deficiency results in female infertility by disturbing steroid pathway and decreasing ovarian reserve in mice. | ||
