NSFL1C Polyclonal antibody

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• NSFL1C Polyclonal antibody (15620-1-AP)

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• KD/KO Validated

NSFL1C Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15620-1-AP Copied!

Publications (6)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

NSFL1 cofactor p47, p97 cofactor p47, UBX domain-containing protein 2C, UBXN2C

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  WB analysis using 15620-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 15620-1-AP

  WB result of NSFL1C antibody (15620-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSFL1C transfected A431 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 15620-1-AP

  Mouse liver tissue was subjected to SDS-PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 15620-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 15620-1-AP

  IP result of anti-NSFL1C (IP:15620-1-AP, 4ug; Detection:15620-1-AP 1:1000) with HEK-293 cells lysate 2640ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse liver tissue, A431 cells, HeLa cells, U-251 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lung cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15620-1-AP targets NSFL1C in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8040

  Product name: Recombinant human NSFL1C protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-370 aa of BC002801

  Sequence: MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQAENEAKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFILMTTFPNKELADESQTLKEANLLNAVIVQRLT

• Full Name: NSFL1 (p97) cofactor (p47)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC002801
• Gene Symbol: NSFL1C
• Gene ID (NCBI): 55968
• RRID: AB_2878158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UNZ2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

NSFL1C Polyclonal antibody (Cat# 15620-1-AP) has 6 citations published in Cancer Research, PNAS, Cell Chemical Biology, Journal of Biological Chemistry, Journal of Cell Science, and Evidence-Based Complementary and Alternative Medicine. Research fields span bladder cancer, VCP/p97 mutations, IFITM3 trafficking, SUMOylation, aggresome formation, and cardiac signaling.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Res; A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer; Authors - Yanchao Yu
human	WB	Proc Natl Acad Sci U S A; Altered cofactor regulation with disease-associated p97/VCP mutations.; Authors - Xiaoyi Zhang
human	WB	Cell Chem Biol; Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.; Authors - Xiaojun Wu
human	WB	J Biol Chem; Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.; Authors - Tao Wang
human	WB,IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli ; KD Validated
mouse	WB	Evid Based Complement Alternat Med; Shenxian-Shengmai Oral Liquid Improves Sinoatrial Node Dysfunction through the PKC/NOX-2 Signaling Pathway.; Authors - Heng Zhang

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for this product reports a 5-star rating. The customer used it for Western Blot on human tissue at a 1:1000 dilution and noted clean, specific bands at the correct molecular weight. Overall, the feedback is positive, highlighting reliable specificity and performance in WB applications.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Julia (Verified Customer) (07-02-2019)

Clean and specific bands on Western blot at correct MW.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human