CoraLite® Plus 647-conjugated NSFL1C Monoclonal antibody

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  IF Staining of A431 using CL647-68182

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 647 NSFL1C antibody (CL647-68182, Clone: 1F12E12 ) at dilution of 1:200.

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  FC experiment of A431 using CL647-68182

  1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated NSFL1C Monoclonal antibody (CL647-68182, Clone:1F12E12)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (1F8D3) (CL647-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

NSFL1C Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL647-68182

Clone No.1F12E12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

UBXN2C, UBX domain-containing protein 2C, p97 cofactor p47, NSFL1 cofactor p47, 1F12E12

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Tested Applications

• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-68182 targets NSFL1C in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8040

  Product name: Recombinant human NSFL1C protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-370 aa of BC002801

  Sequence: MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQAENEAKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFILMTTFPNKELADESQTLKEANLLNAVIVQRLT

• Full Name: NSFL1 (p97) cofactor (p47)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC002801
• Gene Symbol: NSFL1C
• Gene ID (NCBI): 55968
• RRID: AB_3673647
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UNZ2
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.