NSMCE1 Polyclonal antibody

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  WB analysis using 32113-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32113-1-AP (NSMCE1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

NSMCE1 Polyclonal Antibody for WB, ELISA

Cat No. 32113-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

NSE1, Non-structural maintenance of chromosomes element 1 homolog, Non-SMC element 1 homolog, HSPC337, HSPC333

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Tested Applications

• Positive WB detected in: HL-60 cells, HepG2 cells, LNCaP cells, Raji cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32113-1-AP targets NSMCE1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSMCE1 fusion protein Ag36841
• Full Name: non-SMC element 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC018938
• Gene Symbol: NSMCE1
• Gene ID (NCBI): 197370
• RRID: AB_3670196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8WV22
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Non-structural maintenance of chromosomes element 1 homolog (NSMCE1, also known as NSE1) located in the nucleoplasm, which involves the maintenance of genome integrity, DNA damage response, and DNA repair (PMID: 18086888; 20864041; 29225034). NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of the SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair (PMID: 18086888).