NSMCE2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NSMCE2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13627-1-AP

Synonyms

C8orf36, E3 SUMO protein ligase NSE2, hMMS21, MMS21, MMS21 homolog, Non SMC element 2 homolog, NSE2, NSMCE2

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Validation Data Gallery

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  WB analysis using 13627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13627-1-AP (NSMCE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13627-1-AP

  WB result of NSMCE2 antibody (13627-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSMCE2 transfected HeLa cells.

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  IHC staining of mouse testis using 13627-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13627-1-AP (NSMCE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 13627-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13627-1-AP (NSMCE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, U2OS cells
• Positive IHC detected in: mouse testis tissue, mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13627-1-AP targets NSMCE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSMCE2 fusion protein Ag4545
• Full Name: non-SMC element 2, MMS21 homolog (S. cerevisiae)
• Calculated Molecular Weight: 247 aa, 28 kDa
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: BC032797
• Gene Symbol: NSMCE2
• Gene ID (NCBI): 286053
• RRID: AB_10637854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu