Tested Applications
| Positive WB detected in | COLO 320 cells, HepG2 cells, Caco-2 cells, HEK-293 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human oesophagus cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
66580-1-Ig targets NSUN2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NSUN2 fusion protein Ag14791 Predict reactive species |
| Full Name | NOL1/NOP2/Sun domain family, member 2 |
| Calculated Molecular Weight | 767 aa, 86 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC001041 |
| Gene Symbol | NSUN2 |
| Gene ID (NCBI) | 54888 |
| RRID | AB_2881940 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q08J23 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NSUN2 antibody 66580-1-Ig | Download protocol |
| IHC protocol for NSUN2 antibody 66580-1-Ig | Download protocol |
| IF protocol for NSUN2 antibody 66580-1-Ig | Download protocol |
| IP protocol for NSUN2 antibody 66580-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis. | ||
Acta Neuropathol RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation | ||
Cell Metab NSUN2 is a glucose sensor suppressing cGAS/STING to maintain tumorigenesis and immunotherapy resistance | ||
Cell Rep The m5C methyltransferase NSUN2 promotes progression of acute myeloid leukemia by regulating serine metabolism |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Keerthi (Verified Customer) (07-23-2025) | The antibody works very well at the recommended dilutions and performs excellently in both immunoprecipitation and immunoblotting applications. It shows strong specificity and clean bands with minimal background. We also observed consistent results across different batches, which adds to its reliability. Overall, this antibody is a valuable tool for studying target protein interactions and expression levels in our experiments.
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