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NSUN2 Monoclonal antibody
NSUN2 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2H5A2
Cat no : 66580-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | COLO 320 cells, HepG2 cells, Caco-2 cells, HEK-293 cells, Jurkat cells |
Positive IHC detected in | human oesophagus cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
IF | See 1 publications below |
Product Information
66580-1-Ig targets NSUN2 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | NSUN2 fusion protein Ag14791 |
Full Name | NOL1/NOP2/Sun domain family, member 2 |
Calculated molecular weight | 767 aa, 86 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC001041 |
Gene symbol | NSUN2 |
Gene ID (NCBI) | 54888 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for NSUN2 antibody 66580-1-Ig | Download protocol |
IHC protocol for NSUN2 antibody 66580-1-Ig | Download protocol |
IF protocol for NSUN2 antibody 66580-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis. | ||
Acta Neuropathol RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation |