CoraLite® Plus 488-conjugated NSUN2 Monoclonal antibody

• 

  IF Staining of HepG2 using CL488-66580

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66580 (NSUN2 antibody) at dilution of 1:100.

• Featured Product
• KD/KO Validated

NSUN2 Monoclonal Antibody for IF/ICC

Cat No. CL488-66580

Clone No.2H5A2

Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

IF/ICC

Synonyms

FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-66580 targets NSUN2 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14791

  Product name: Recombinant human NSUN2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 415-767 aa of BC001041

  Sequence: HHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR

• Full Name: NOL1/NOP2/Sun domain family, member 2
• Calculated Molecular Weight: 767 aa, 86 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC001041
• Gene Symbol: NSUN2
• Gene ID (NCBI): 54888
• RRID: AB_2883358
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q08J23
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nucleic Acids Res; Spatial regulation of NSUN2-mediated tRNA m5C installation in cognitive function; Authors - Yulia Gonskikh