CoraLite® Plus 488-conjugated NSUN2 Monoclonal antibody

NSUN2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H5A2

Cat no : CL488-66580

Synonyms

FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66580

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66580 (NSUN2 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66580 targets NSUN2 in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NSUN2 fusion protein Ag14791
• Full Name: NOL1/NOP2/Sun domain family, member 2
• Calculated Molecular Weight: 767 aa, 86 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC001041
• Gene Symbol: NSUN2
• Gene ID (NCBI): 54888
• RRID: AB_2883358
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.