NSUN4 Recombinant monoclonal antibody, PBS Only

• 

  IF Staining of HepG2 using 84275-3-RR (same clone as 84275-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN4 antibody (84275-3-RR, Clone: 241115G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84275-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84275-3-RR (same clone as 84275-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN4 antibody (84275-3-RR, Clone: 241115G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84275-3-PBS in a different storage buffer formulation.

NSUN4 Uni-rAb^® Recombinant Antibody for IF/ICC, Indirect ELISA

Cat No. 84275-3-PBS

Clone No.241115G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Synonyms

RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 241115G11

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Product Information

84275-3-PBS targets NSUN4 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag31828

  Product name: Recombinant human NSUN4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 26-284 aa of BC014441

  Sequence: RYKKKWAATEPKFPAVRLALQNFDMAYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQIL

• Full Name: NOL1/NOP2/Sun domain family, member 4
• Calculated Molecular Weight: 384 aa, 43 kDa
• GenBank Accession Number: BC014441
• Gene Symbol: NSUN4
• Gene ID (NCBI): 387338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96CB9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly.