CoraLite® Plus 488-conjugated NSUN4 Recombinant monoclonal antibody

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  IF Staining of HepG2 using CL488-84275-3

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 NSUN4 antibody (CL488-84275-3, Clone: 241115G11 ) at dilution of 1:200.

NSUN4 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-84275-3

Clone No.241115G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

241115G11, 5-methylcytosine rRNA methyltransferase NSUN4, 5-methylcytosine tRNA methyltransferase NSUN4, RP4 603I14.2

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84275-3 targets NSUN4 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag31828

  Product name: Recombinant human NSUN4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 26-284 aa of BC014441

  Sequence: RYKKKWAATEPKFPAVRLALQNFDMAYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQIL

• Full Name: NOL1/NOP2/Sun domain family, member 4
• Calculated Molecular Weight: 384 aa, 43 kDa
• GenBank Accession Number: BC014441
• Gene Symbol: NSUN4
• Gene ID (NCBI): 387338
• RRID: AB_3673350
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96CB9
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly.