NT5DC1 Polyclonal antibody, PBS Only

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• NT5DC1 Polyclonal antibody, PBS Only (24102-1-PBS)

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  WB analysis of MCF-7 using 24102-1-AP (same clone as 24102-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 24102-1-AP (same clone as 24102-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 24102-1-AP (same clone as 24102-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 24102-1-AP (same clone as 24102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 24102-1-AP (same clone as 24102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 24102-1-AP (same clone as 24102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 24102-1-AP (same clone as 24102-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 24102-1-AP (same clone as 24102-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NT5DC1 antibody (24102-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 24102-1-PBS in a different storage buffer formulation.

NT5DC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 24102-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NT5C2L1, EC:3.1.3.-, 5'-nucleotidase domain-containing protein 1

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Product Information

24102-1-PBS targets NT5DC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20916

  Product name: Recombinant human NT5DC1 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-297 aa of BC015138

  Sequence: WKDIVAAIQHNYKMSAFKENCGIYFPEIKRDPGRYLHSCPESVKKWLRQLKNAGKILLLITSSHSDYCRLLCEYILGNDFTDLFDIVITNALKPGFFSHLPSQRPFRTLENDEEQEALPSLDKPGWYSQGNAVHLYELLKKMTGKPEPKVVYFGDSMHSDIFPARHYSNWETVLILEELRGDEGTRSQRPEESEPLEKKGKYEGPKAKPLNTSSKKWGSFFIDSVLGLENTEDSLVYTWSCKRISTYSTIAIPSIEAIAELPLDYKFTRFSSSNSKTAGYYPNPPLVLSSDETLISK

• Full Name: 5'-nucleotidase domain containing 1
• Calculated Molecular Weight: 455 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC015138
• Gene Symbol: NT5DC1
• Gene ID (NCBI): 221294
• RRID: AB_2879431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q5TFE4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.