NUBP2 Polyclonal antibody

NUBP2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15409-1-AP

Synonyms

CFD1, NBP 2, NUBP1, NUBP2, Nucleotide binding protein 2



Tested Applications

Positive WB detected inL02 cells, HepG2 cells, mouse pancreas tissue, NIH/3T3 cells
Positive IHC detected inhuman skeletal muscle tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15409-1-AP targets NUBP2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUBP2 fusion protein Ag7676
Full Name nucleotide binding protein 2 (MinD homolog, E. coli)
Calculated molecular weight 29 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC002768
Gene symbol NUBP2
Gene ID (NCBI) 10101
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NUBP2 antibody 15409-1-APDownload protocol
IHC protocol for NUBP2 antibody 15409-1-APDownload protocol
IF protocol for NUBP2 antibody 15409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.

Authors - Nima N Naseri