NUBP2 Polyclonal antibody

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  WB analysis using 15409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15409-1-AP (NUBP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15409-1-AP (NUBP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15409-1-AP (NUBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15409-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15409-1-AP (NUBP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 15409-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15409-1-AP (NUBP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 15409-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15409-1-AP (NUBP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 15409-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15409-1-AP (NUBP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 15409-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15409-1-AP (NUBP2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of L02 using 15409-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed L02 cells using NUBP2 antibody (15409-1-AP) at dilution of 1:200 and Cy3-conjugated Goat Anti-Rabbit IgG(H+L).

NUBP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15409-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Nucleotide-binding protein 2, Nucleotide binding protein 2, NUBP1, NBP 2, Cytosolic Fe-S cluster assembly factor NUBP2

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Tested Applications

• Positive WB detected in: HeLa cells, mouse pancreas tissue, L02 cells, U-251 cells, HepG2 cells, NIH/3T3 cells
• Positive IHC detected in: human skeletal muscle tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

15409-1-AP targets NUBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7676

  Product name: Recombinant human NUBP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-271 aa of BC002768

  Sequence: MEAAAEPGNLAGVRHIILVLSGKGGVGKSTISTELALALRHAGKKVGILDVDLCGPSIPRMLGAQGRAVHQCDRGWAPVFLDREQSISLMSVGFLLEKPDEAVVWRGPKKNALIKQFVSDVAWGELDYLVVDTPPGTSDEHMATIEALRPYQPLGALVVTTPQAVSVGDVRRELTFCRKTGLRVMGIVENMSGFTCPHCTECTSVFSRGGGEELAQLAGVPFLGSVPLDPALMRTLEEGHDFIQEFPGSPAFAALTSIAQKILDATPACLP

• Full Name: nucleotide binding protein 2 (MinD homolog, E. coli)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC002768
• Gene Symbol: NUBP2
• Gene ID (NCBI): 10101
• RRID: AB_10858630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5Y2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Struct Mol Biol; Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.; Authors - Nima N Naseri
human	WB	J Biol Chem; Iron-Regulated Assembly of the Cytosolic Iron-Sulfur Cluster Biogenesis Machinery.; Authors - Xiaorui Fan
human,mouse	WB,IHC	Mol Cell Biochem; Abnormal upregulation of NUBP2 contributes to cancer progression in colorectal cancer; Authors - Danfeng Lan