NUDCD1 Polyclonal antibody

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  WB analysis using 32473-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32473-1-AP (NUDCD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 32473-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NUDCD1 antibody (32473-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

NUDCD1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 32473-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Tumor antigen CML66, NudC domain-containing protein 1, CML66, Chronic myelogenous leukemia tumor antigen 66

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Tested Applications

• Positive WB detected in: MCF-7 cells, MDA-MB-231 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32473-1-AP targets NUDCD1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38518

  Product name: Recombinant human NUDCD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 281-498 aa of NM_032869.3

  Sequence: QQTEDDLTVTIRLPEDSTKEDIQIQFLPDHINIVLKDHQFLEGKLYSSIDHESSTWIIKESNSLEISLIKKNEGLTWPELVIGDKQGELIRDSAQCAAIAERLMHLTSEELNPNPDKEKPPCNAQELEECDIFFEESSSLCRFDGNTLKTTHVVNLGSNQYLFSVIVDPKEMPCFCLRHDVDALLWQPHSSKQDDMWEHIATFNALGYVQASKRDKKF

• Full Name: NudC domain containing 1
• Calculated Molecular Weight: 67 kDa，583aa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: NM_032869.3
• Gene Symbol: NUDCD1
• Gene ID (NCBI): 84955
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96RS6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NudC domain-containing protein 1 (NUDCD1, also known as CML66) is a protein that plays a crucial role in various biological processes, including cell proliferation, migration, cell cycle progression, and intracellular material transport (PMID: 38553373). NUDCD1 has been identified as an oncoprotein frequently activated or overexpressed in multiple human cancers, suggesting its role in promoting cancer cell growth and metastasis (PMID: 29888104). It is abnormally activated in various tumors and has been identified as a cancer antigen (PMID: 37338527).