Various lysates were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21205-1-AP
WB result of NUDCD2 antibody (21205-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDCD2 transfected HEK-293 cells.
WB result of NUDCD2 antibody (21205-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDCD2 transfected HEK-293 cells.
WB analysis of human brain using 21205-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21205-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21205-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 21205-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human kidney tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 21205-1-AP
IP result of anti-NUDCD2 (IP:21205-1-AP, 4ug; Detection:21205-1-AP 1:500) with HepG2 cells lysate 1080 ug.
Immunohistochemical analysis of paraffin-embedded human kidney using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 21205-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21205-1-AP (NUDCD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21205-1-AP (NUDCD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 21205-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 10x lens).
Immunofluorescent analysis of HepG2 cells, using NUDCD2 antibody 21205-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HepG2 using 21205-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 21205-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 21205-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HeLa using 21205-1-AP
Immunofluorescent analysis of Hela cells, using NUDCD2 antibody 21205-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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HepG2 cells, HEK-293 cells, human brain tissue, human kidney tissue, mouse brain tissue, rat brain tissue
Positive IP detected in
HepG2 cells
Positive IHC detected in
human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells, HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:12000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:200-1:800
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715).
Various lysates were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21205-1-AP
WB result of NUDCD2 antibody (21205-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDCD2 transfected HEK-293 cells.
WB analysis of human brain using 21205-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21205-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21205-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 21205-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human kidney using 21205-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 21205-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 21205-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21205-1-AP (NUDCD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 21205-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human breast cancer using 21205-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of HepG2 using 21205-1-AP
IP result of anti-NUDCD2 (IP:21205-1-AP, 4ug; Detection:21205-1-AP 1:500) with HepG2 cells lysate 1080 ug.
IF/ICC Figures
IF Staining of HepG2 using 21205-1-AP
Immunofluorescent analysis of HepG2 cells, using NUDCD2 antibody 21205-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HepG2 using 21205-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 21205-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 21205-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HeLa using 21205-1-AP
Immunofluorescent analysis of Hela cells, using NUDCD2 antibody 21205-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
NUDCD2 Polyclonal antibody
Catalog Number
21205-1-AP
Citations
1
Dilutions
WB : 1:2000-1:12000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
