Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human kidney tissue, human rectal cancer tissue, mouse lung tissue, mouse stomach tissue, mouse skeletal muscle tissue, human stomach cancer tissue, mouse adrenal gland tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12889-1-AP targets NUDT16 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3925 Product name: Recombinant human NUDT16 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-195 aa of BC031215 Sequence: MAGARRLELGEALALGSGWRHVCHALLYAPDPGMLFGRIPLRYAILMQMRFDGRLGFPGGFVDTQDRSLEDGLNRELREELGEAAAAFRVERTDYRSSHVGSGPRVVAHFYAKRLTLEELLAVEAGATRAKDHGLEVLGLVRVPLYTLRDGVGGLPTFLENSFIGSAREQLLEALQDLGLLQSGSISGLKIPAHH Predict reactive species |
| Full Name | nudix (nucleoside diphosphate linked moiety X)-type motif 16 |
| Calculated Molecular Weight | 195 aa, 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC031215 |
| Gene Symbol | NUDT16 |
| Gene ID (NCBI) | 131870 |
| RRID | AB_10640536 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96DE0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NUDT16 antibody 12889-1-AP | Download protocol |
| IHC protocol for NUDT16 antibody 12889-1-AP | Download protocol |
| IP protocol for NUDT16 antibody 12889-1-AP | Download protocol |
| WB protocol for NUDT16 antibody 12889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NUDT16 Polyclonal antibody (Cat# 12889-1-AP) has 9 citations. Journals include Cancer Research, Redox Biology, Molecular Cell, Nucleic Acids Research, PNAS, Frontiers in Immunology, Molecular and Cellular Biochemistry, and Molecular Biology of the Cell. Research fields span DNA double-strand break repair, PARylation regulation, cancer biology (colorectal and ovarian), ferroptosis, RNA quality control, m7G modification, and innate immune signaling.
| Species | Application | Title |
|---|---|---|
Cancer Res Nudix hydrolase NUDT16 regulates 53BP1 protein by reversing 53BP1 ADP-ribosylation. | ||
Redox Biol Overexpression of NUDT16L1 sustains proper function of mitochondria and leads to ferroptosis insensitivity in colorectal cancer
| ||
Mol Cell 5'-Triphosphate guanosine RNAs recruit GTP-binding proteins to suppress RIG-I/IFN type I signaling.
| ||
Nucleic Acids Res NUDT16 regulates CtIP PARylation to dictate homologous recombination repair
| ||
Proc Natl Acad Sci U S A Quality control of assembly-defective U1 snRNAs by decapping and 5'-to-3' exonucleolytic digestion.
| ||
Front Immunol m7G regulator-mediated methylation modification patterns define immune cell infiltration and patient survival |
