NUMB Recombinant antibody

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  WB analysis using 85939-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85939-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 85939-4-RR

  IP result of anti-NUMB (IP:85939-4-RR, 4ug; Detection:85939-4-RR 1:3000) with HeLa cells lysate 1600 ug.

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  IHC staining of human breast cancer using 85939-4-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 85939-4-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85939-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85939-4-RR against Human NUMB were performed. The affinity constant is 1.15 nM.

NUMB Uni-rAb^TM Recombinant Antibody for WB, IHC, IP, ELISA

Cat No. 85939-4-RR

Clone No.250417F6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

C14orf41, h Numb, h-Numb

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Tested Applications

• Positive WB detected in: A431 cells, A-204 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, SKOV-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85939-4-RR targets NUMB in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NUMB fusion protein Ag31553
• Full Name: numb homolog (Drosophila)
• Calculated Molecular Weight: 71KD
• Observed Molecular Weight: 66-71 kDa
• GenBank Accession Number: NM_001005743
• Gene Symbol: NUMB
• Gene ID (NCBI): 8650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49757
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene.