gp210 Polyclonal antibody

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  WB analysis of mouse lung using 16909-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16909-1-AP (gp210 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16909-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16909-1-AP (gp210 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 16909-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16909-1-AP (gp210 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16909-1-AP

  IP result of anti-gp210 (IP:16909-1-AP, 4ug; Detection:16909-1-AP 1:300) with HeLa cells lysate 3040 ug.

gp210 Polyclonal Antibody for WB, IP, ELISA

Cat No. 16909-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

gp210, KIAA0906, Nuclear pore protein gp210, nucleoporin 210kDa, Nucleoporin Nup210, NUP210, POM210

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse thymus tissue, MCF-7 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16909-1-AP targets gp210 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10533

  Product name: Recombinant human NUP210 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 10-351 aa of BC067089

  Sequence: LLTLSVLLAAGPSAAAAKLNIPKVLLPFTRATRVNFTLEASEGCYRWLSTRPEVASIEPLGLDEQQCSQKAVVQARLTQPARLTSIIFAEDITTGQVLRCDAIVDLIHDIQIVSTTRELYLEDSPLELKIQALDSEGNTFSTLAGLVFEWTIVKDSEADRFSDSHNALRILTFLESTYIPPSYISEMEKAAKQGDTILVSGMKTGSSKLKARIQEAVYKNVRPAEVRLLILENILLNPAYDVYLMVGTSIHYKVQKIRQGKITELSMPSDQYELQLQNSIPGPEGDPARPVAVLAQDTSMVTALQLGQSSLVLGHRSIRMQGASRLPNSTIYVVEPGYLGFT

• Full Name: nucleoporin 210kDa
• Calculated Molecular Weight: 967aa,106 kDa; 1887aa,205 kDa
• Observed Molecular Weight: 205 kDa
• GenBank Accession Number: BC067089
• Gene Symbol: gp210
• Gene ID (NCBI): 23225
• RRID: AB_2157365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TEM1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.