NUP50 Monoclonal antibody, PBS Only

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  WB analysis using 67001-1-Ig (same clone as 67001-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67001-1-Ig (same clone as 67001-1-PBS)

  WB result of NUP50 antibody (67001-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUP50 transfected HEK-293 cells. This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67001-1-Ig (same clone as 67001-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67001-1-Ig (same clone as 67001-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67001-1-Ig (same clone as 67001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67001-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NUP50 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67001-1-PBS

Clone No.1D5A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

1D5A7, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, nucleoporin 50kDa, Nucleoporin Nup50

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Product Information

67001-1-PBS targets NUP50 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15144

  Product name: Recombinant human NUP50 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 141-440 aa of BC028125

  Sequence: DWIVKHVNTNPLCDLTPIFKDYEKYLANIEQQHGNSGRNSESESNKVAAETQSPSLFGSTKLQQESTFLFHGNKTEDTPDKKMEVASEKKTDPSSLGATSASFNFGKKVDSSVLGSLSSVPLTGFSFSPGNSSLFGKDTTQSKPVSSPFPTKPLEGQAEGDSGECKGGDEEENDEPPKVVVTEVKEEDAFYSKKCKLFYKKDNEFKEKGIGTLHLKPTANQKTQLLVRADTNLGNILLNVLIPPNMPCTRTGKNNVLIVCVPNPPIDEKNATMPVTMLIRVKTSEDADELHKILLEKKDA

• Full Name: nucleoporin 50kDa
• Calculated Molecular Weight: 468 aa, 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC028125
• Gene Symbol: NUP50
• Gene ID (NCBI): 10762
• RRID: AB_2882318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UKX7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L.