Tested Applications
| Positive WB detected in | HCT 116 cells, HeLa cells, HEK-293 cells, Ramos cells, Daudi cels, Sp2/0 cells, MCF-7 cells, HT-29 cells, U2OS cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67001-1-Ig targets NUP50 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15144 Product name: Recombinant human NUP50 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 141-440 aa of BC028125 Sequence: DWIVKHVNTNPLCDLTPIFKDYEKYLANIEQQHGNSGRNSESESNKVAAETQSPSLFGSTKLQQESTFLFHGNKTEDTPDKKMEVASEKKTDPSSLGATSASFNFGKKVDSSVLGSLSSVPLTGFSFSPGNSSLFGKDTTQSKPVSSPFPTKPLEGQAEGDSGECKGGDEEENDEPPKVVVTEVKEEDAFYSKKCKLFYKKDNEFKEKGIGTLHLKPTANQKTQLLVRADTNLGNILLNVLIPPNMPCTRTGKNNVLIVCVPNPPIDEKNATMPVTMLIRVKTSEDADELHKILLEKKDA Predict reactive species |
| Full Name | nucleoporin 50kDa |
| Calculated Molecular Weight | 468 aa, 50 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC028125 |
| Gene Symbol | NUP50 |
| Gene ID (NCBI) | 10762 |
| RRID | AB_2882318 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UKX7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NUP50 antibody 67001-1-Ig | Download protocol |
| WB protocol for NUP50 antibody 67001-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NUP50 Monoclonal antibody (1D5A7), catalog number 67001-1-Ig, has 1 total citation. The citation appears in bioRxiv (2025), a preprint server. The associated research field is plant cell biology, specifically investigating RNA splicing's role in abscission and its modulation by the abscission checkpoint. The cited application was immunofluorescence (IF) in human samples.
| Species | Application | Title |
|---|---|---|
bioRxiv RNA splicing is required for timely completion of abscission and is modulated by the abscission checkpoint.
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