Tested Applications
| Positive WB detected in | mouse kidney tissue, HeLa cells, mouse lung tissue, mouse thymus tissue, rat kidney tissue, rat thymus tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16970-1-AP targets NVL in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9205 Product name: Recombinant human NVL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 309-659 aa of BC012105 Sequence: LPSKGVQEERLGTEPTSETQDELQRLLGLLRDQDPLSEEQMQGLCIELNDFIVALSSVQPSAKREGFVTVPNVTWADIGALEDIREELTMAILAPVRNPDQFKALGLVTPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRRSDRETGASVRVVNQLLTEMDGLEARQQVFIMAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRLAILKTITKNGTKPPLDADVNLEAIAGDLRCDCYTGADLSALVREASICALRQEMARQKSGNEKGELKVSHKHFEEAFKKVRSSISKKDQIMYERLQESLSR Predict reactive species |
| Full Name | nuclear VCP-like |
| Calculated Molecular Weight | 856aa,95 kDa; 659aa,73 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | BC012105 |
| Gene Symbol | NVL |
| Gene ID (NCBI) | 4931 |
| RRID | AB_2157811 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15381 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NVL antibody 16970-1-AP | Download protocol |
| WB protocol for NVL antibody 16970-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NVL Polyclonal antibody (catalog number 16970-1-AP) has 6 total citations. These appear in Nature, Nature Communications, Journal of Experimental & Clinical Cancer Research, Journal of Cell Biology, RMD Open, and BMC Medical Genomics. The associated research fields include ribosome inventory during nutrient stress, nucleolar protein localization and SUMO pathway regulation, gastric cancer progression, autoantibody detection (ELISA), and prostate cancer prognostic modeling.
| Species | Application | Title |
|---|---|---|
Nat Commun Brucella effectors NyxA and NyxB target SENP3 to modulate the subcellular localisation of nucleolar proteins | ||
J Exp Clin Cancer Res SNORA37/CMTR1/ELAVL1 feedback loop drives gastric cancer progression via facilitating CD44 alternative splicing | ||
J Cell Biol Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteases. | ||
RMD Open Development of an enzyme-linked immunosorbent assay for the efficient detection of autoantibodies against nuclear valosin-containing protein-like protein (NVL) 2 using its manipulated cDNA. | ||
BMC Med Genomics Construction and verification of a prognostic model for prostate cancer based on ribosome biogenesis-related genes. |
Reviews
What customers say
The single review for this antibody (rated 4/5) reports use in Western Blot on HEK293T cells at a 1:1000 dilution. The reviewer notes the antibody performs adequately, though a few nonspecific bands appear. They were able to identify the correct target band by performing nuclear/cytosolic fractionation, consistent with NVL's expected localization in both compartments for ribosome shuttling. Overall, the antibody is considered functional with minor background issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
H (Verified Customer) (04-05-2020) | This antibody works okay. There are a few nonspecific bands, but I could tell which is the target band because I performed nuclear cytosolic fractionation. NVL is supposed to be in both nucleus and cytosol to shuttle ribosomes.
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