CoraLite® Plus 488-conjugated NXF1 Monoclonal antibody

NXF1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C2H1

Cat no : CL488-67914

Synonyms

DKFZp667O0311, MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-67914

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 NXF1 antibody (CL488-67914, Clone: 2C2H1 ) at dilution of 1:200.

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  FC experiment of HEK-293 using CL488-67914

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NXF1 (CL488-67914, Clone:2C2H1) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: HEK-293 cells
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67914 targets NXF1 in IF, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NXF1 fusion protein Ag27329
• Full Name: nuclear RNA export factor 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC004904
• Gene Symbol: NXF1
• Gene ID (NCBI): 10482
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.