NXN Monoclonal antibody, PBS Only

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  WB analysis using 66603-1-Ig (same clone as 66603-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66603-1-Ig (NXN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66603-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66603-1-Ig (same clone as 66603-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66603-1-Ig (NXN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66603-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66603-1-Ig (same clone as 66603-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66603-1-Ig (NXN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66603-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66603-1-Ig (same clone as 66603-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NXN antibody (66603-1-Ig, Clone: 2C6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66603-1-PBS in a different storage buffer formulation.

NXN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66603-1-PBS

Clone No.2C6A4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:1.8.1.8, NRX, Nucleoredoxin, TRG 4

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Product Information

66603-1-PBS targets NXN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9248

  Product name: Recombinant human NXN protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-326 aa of BC009327

  Sequence: ADVSLHRNPATLKLWNKYRISNIPSLIFLDATTGKVVCRNGLLVIRDDPEGLEFPWGPKPFREVIAGPLLRNNGQSLESSSLEGSHVGVYFSAHWCPPCRSLTRVLVESYRKIKEAGQNFEIIFVSADRSEESFKQYFSEMPWLAVPYTDEARRSRLNRLYGIQGIPTLIMLDPQGEVITRQGRVEVLNDEDCREFPWHPKPVLELSDSNAAQLNEGPCLVLFVDSEDDGESEAAKQLIQPIAEKIIAKYKAKEEEAPLLFFVAGEDDMTDSLRDYTNLPEAAPLLTILDMSARAKYVMDVEEITPAIVEAFVNDFLAEKLKPEPI

• Full Name: nucleoredoxin
• Calculated Molecular Weight: 435 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC009327
• Gene Symbol: NXN
• Gene ID (NCBI): 64359
• RRID: AB_2881963
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6DKJ4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.