NXN Monoclonal antibody

NXN Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human, Mouse , Rat







Cat no : 66603-1-Ig


NRX, nucleoredoxin, NXN, TRG 4

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66603-1-Ig targets NXN in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NXN fusion protein Ag9248
Full Name nucleoredoxin
Calculated molecular weight 435 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC009327
Gene symbol NXN
Gene ID (NCBI) 64359
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.


Product Specific Protocols
WB protocol for NXN antibody 66603-1-IgDownload protocol
IHC protocol for NXN antibody 66603-1-IgDownload protocol
IF protocol for NXN antibody 66603-1-IgDownload protocol
Standard Protocols
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