NXN Monoclonal antibody

NXN Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2C6A4

Cat no : 66603-1-Ig

Synonyms

NRX, nucleoredoxin, NXN, TRG 4



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, HSC-T6
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66603-1-Ig targets NXN in WB, IHC,ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NXN fusion protein Ag9248
Full Name nucleoredoxin
Calculated molecular weight 435 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC009327
Gene symbol NXN
Gene ID (NCBI) 64359
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.

Protocols

Product Specific Protocols
WB protocol for NXN antibody 66603-1-IgDownload protocol
IHC protocol for NXN antibody 66603-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols