Nectin-4/PVRL4 Recombinant monoclonal antibody

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  IHC staining of human placenta using 83223-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83223-4-RR (Nectin-4/PVRL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 83223-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83223-4-RR (Nectin-4/PVRL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 83223-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Nectin-4/PVRL4 antibody (83223-4-RR, Clone: 240009F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of MCF-7 using 83223-4-RR

  1x10^6 MCF-7 cells were surface stained with 0.25 ug Nectin-4/PVRL4 Recombinant Antibody (83223-4-RR, Clone: 240009F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 83223-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83223-4-RR against Human Nectin-4/PVRL4 were performed. The affinity constant is below 1 pM.

Nectin-4/PVRL4 Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, FC, ELISA

Cat No. 83223-4-RR

Clone No.240009F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC, ELISA

Synonyms

Nectin-4, PVRL4, 240009F2, Ig superfamily receptor LNIR, LNIR

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Tested Applications

• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83223-4-RR targets Nectin-4/PVRL4 in IHC, IF/ICC, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Affinity:
  K_D=2.28 x 10^-10M
  K_Off=2.98 x 10^-5M
  K_On=2.62 x 10^5M
• Immunogen:

  CatNo: Eg0415

  Product name: Recombinant human nectin 4 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 32-349 aa of BC010423

  Sequence: GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSAS

• Full Name: poliovirus receptor-related 4
• Calculated Molecular Weight: 510 aa, 55 kDa
• GenBank Accession Number: BC010423
• Gene Symbol: Nectin-4
• Gene ID (NCBI): 81607
• RRID: AB_3670907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96NY8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma. Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).