Neurabin 2 Polyclonal antibody, PBS Only

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• Neurabin 2 Polyclonal antibody, PBS Only (55129-1-PBS)

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  WB analysis of mouse brain using 55129-1-AP (same clone as 55129-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55129-1-AP (Neurabin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55129-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 55129-1-AP (same clone as 55129-1-PBS)

  WB result of PPP1R9B antibody (55129-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Neurabin 2 transfected A549 cells. This data was developed using the same antibody clone with 55129-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 55129-1-AP (same clone as 55129-1-PBS)

  IP result of anti-Neurabin 2 (IP:55129-1-AP, 4ug; Detection:55129-1-AP 1:300) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 55129-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55129-1-AP (same clone as 55129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 55129-1-AP (Neurabin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55129-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 55129-1-AP (same clone as 55129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 55129-1-AP (Neurabin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55129-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Neurabin 2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 55129-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

55129-1-PBS targets Neurabin 2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 9B
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: NM_032595
• Gene Symbol: Spinophilin/Neurabin 2
• Gene ID (NCBI): 84687
• RRID: AB_10837358
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96SB3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Neurabin 2, also named as Spinophilin, seems to act as a scaffold protein in multiple signaling pathways. It modulates excitatory synaptic transmission and dendritic spine morphology. PPP1R9B binds to actin filaments (F-actin) and shows cross-linking activity. It may play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. PPP1R9B plays a role in regulation of G-protein coupled receptor signaling, including D2Rs and alpha-adrenergic receptors. PPP1R9B probably regulates p70 S6 kinase activity by forming a complex with TIAM. The antibody is specific to PPP1R9B. The predicted molecular weight of spinophilin is 89 kDa, which differs significantly from the apparent MW seen in SDS/PAGE. Both the expressed full-length cDNA and the endogenous protein run at 120-130 kDa. This may be due to an extended conformation and/or low SDS binding capacity. (PMID: 28941770, PMID: 9275233).